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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2022.12
- 수록면
- 656 - 664 (9page)
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이 논문의 연구 히스토리 (2)
2022
A New Approach Using the SYBR Green-Based Real-Time PCR Method for Detection of Soft Rot Pectobacterium odoriferum Associated with Kimchi Cabbage
Yong Ju Jin
,
Dawon Jo
,
Soon-Wo Kwon
외 4명
The Plant Pathology Journal
2022.12
학술저널
A New Approach Using the SYBR Green-based Real-Time PCR Method for Detection of Soft Rot Pectobacterium odoriferum Associated with Kimchi Cabbage
Yong Ju Jin
,
Dawon Jo
,
Yu-na Jeon
외 4명
한국미생물학회 학술대회논문집
2022.10
학술대회자료
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초록· 키워드
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Pectobacterium odoriferum is the primary causative agent in Kimchi cabbage soft-rot diseases. The pathogenic bacteria Pectobacterium genera are responsible for significant yield losses in crops. However, P. odoriferum shares a vast range of hosts with P. carotovorum, P. versatile, and P. brasiliense, and has similar biochemical, phenotypic, and genetic characteristics to these species. Therefore, it is essential to develop a P. odoriferumspecific diagnostic method for soft-rot disease because of the complicated diagnostic process and management as described above. Therefore, in this study, to select P. odoriferum-specific genes, species-specific genes were selected using the data of the P. odoriferum JK2.1 whole genome and similar bacterial species registered with NCBI. Thereafter, the specificity of the selected gene was tested through blast analysis. We identified novel species-specific genes to detect and quantify targeted P. odoriferum and designed specific primer sets targeting HAD family hydrolases. It was confirmed that the selected primer set formed a specific amplicon of 360 bp only in the DNA of P. odoriferum using 29 Pectobacterium species and related species. Furthermore, the population density of P. odoriferum can be estimated without genomic DNA extraction through SYBR Green-based real-time quantitative PCR using a primer set in plants. As a result, the newly developed diagnostic method enables rapid and accurate diagnosis and continuous monitoring of soft-rot disease in Kimchi cabbage without additional procedures from the plant tissue.
목차
Materials and Methods
Results
Discussion
References
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A New Approach Using the SYBR Green-based Real-Time PCR Method for Detection of Soft Rot Pectobacterium odoriferum Associated with Kimchi Cabbage
한국미생물학회 학술대회논문집
2022 .10
-
Antibiotic Resistance of Pectobacterium Korean Strains Susceptible to the Bacteriophage phiPccP-1
Research in Plant Disease(식물병연구)
2022 .09
-
[Erratum] First Report of Pectobacterium versatile as the Causal Pathogen of Soft Rot in Kimchi Cabbage in Korea
Research in Plant Disease(식물병연구)
2023 .06
-
Distribution of Pectobacterium Species Isolated in South Korea and Comparison of Temperature Effects on Pathogenicity
The Plant Pathology Journal
2020 .08
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Genome Wide Analysis of the Potato Soft Rot Pathogen Pectobacterium carotovorum Strain ICMP 5702 to Predict Novel Insights into Its Genetic Features
The Plant Pathology Journal
2022 .04
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First Report of Soft Rot by Pectobacterium carotovorum subsp. brasiliense on Amaranth in Korea
Research in Plant Disease(식물병연구)
2018 .12
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First Report of Melon Soft Rot Disease Caused by Pectobacterium brasiliense in Korea
Research in Plant Disease(식물병연구)
2023 .09
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First Report of Pectobacterium versatile as the Causal Pathogen of Soft Rot in Kimchi Cabbage in Korea
Research in Plant Disease(식물병연구)
2023 .03
-
Assessment of the resistance of bacterial soft rot caused by Pectobacterium carotovorum subsp. carotovorum KACC 21701 in Kimchi cabbage genetic resources
환경생물
2022 .12
-
First Report of Pectobacterium brasiliense Causing Bitter Melon Soft Rot Disease in Korea
Research in Plant Disease(식물병연구)
2023 .12
-
Pectobacterium brasiliense as a Causative Agent for Soft Rot of Radish in Korea
Research in Plant Disease(식물병연구)
2023 .03
-
Erratum: Disruption of the metC Gene Affects Methionine Biosynthesis in Pectobacterium carotovorum subsp. carotovorum Pcc21 and Reduces Soft-Rot Disease
The Plant Pathology Journal
2025 .04
-
First Report of Soft Rot Caused by Pectobacterium brasiliense on Cucumber in Korea
Research in Plant Disease(식물병연구)
2023 .09
-
First Report of Pectobacterium aroidearum Causing Soft Rot on Ficus carica in Korea
Research in Plant Disease(식물병연구)
2024 .03
-
Transcriptional Response of Pectobacterium carotovorum to Cinnamaldehyde Treatment
Journal of Microbiology and Biotechnology
2024 .03
-
Volatile Metabolic Markers for Monitoring Pectobacterium carotovorum subsp. carotovorum Using Headspace Solid-Phase Microextraction Coupled with Gas Chromatography-Mass Spectrometry
Journal of Microbiology and Biotechnology
2021 .01
-
Disruption of the metC Gene Affects Methionine Biosynthesis in Pectobacterium carotovorum subsp. carotovorum Pcc21 and Reduces Soft-Rot Disease
The Plant Pathology Journal
2023 .02
-
A Multiplex-PCR for Rapid Identification of 4 Enterococcus Species Using Species-Specific Primers from Comparative Genomics
한국미생물학회 학술대회논문집
2016 .04
-
Development of a Species-specific PCR Assay for Three Xanthomonas Species, Causing Bulb and Flower Diseases, Based on Their Genome Sequences
The Plant Pathology Journal
2015 .09
-
First Report of Pectobacterium brasiliense Causing Soft Rot on Graft Cactus in Korea
Research in Plant Disease(식물병연구)
2022 .09
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