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논문 기본 정보

자료유형
학술저널
저자정보
Abaineh D. Endalew (Kansas State University) Bonto Faburay (Kansas State University) Jessie D. Trujillo (Kansas State University) Natasha N. Gaudreault (Kansas State University) A. Sally Davis (Kansas State University) Vinay Shivanna (Kansas State University) Sun-Young Sunwoo (Kansas State University) Wenjun Ma (Kansas State University) Barbara S. Drolet (United States Department of Agriculture) D. Scott McVey (United States Department of Agriculture) Igor Morozov (Kansas State University) William C. Wilson (United States Department of Agriculture) Juergen A. Richt (Kansas State University)
저널정보
대한수의학회 Journal of Veterinary Science Journal of Veterinary Science 제20권 제6호
발행연도
2019.11
수록면
1 - 11 (11page)

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The Schmallenberg virus (SBV) is an orthobunyavirus that causes abortions, stillbirths, and congenital defects in pregnant sheep and cattle. Inactivated or live attenuated vaccines have been developed in endemic countries, but there is still interest in the development of SBV vaccines that would allow Differentiating Infected from Vaccinated Animals (DIVA). Therefore, an attempt was made to develop novel DIVA-compatible SBV vaccines using SBV glycoproteins expressed in baculovirus. All vaccines and phosphate buffered saline (PBS) controls were prepared with adjuvant and administered subcutaneously to cattle at 6 month of age. The first trial included 2 groups of animals vaccinated with either carboxyl-terminus glycoprotein (Gc) or PBS and boosted after 2 weeks. In the second trial, 3 groups of cattle were administered either Gc, Gc and amino-terminus glycoprotein (Gn), or PBS with a booster vaccination after 3 weeks. The animals were challenged with SBV 9 days after the booster vaccination in the first study, and 3 weeks after the booster vaccination in the second study. Using a SBV Gc-specific enzyme-linked immunosorbent assay, antibodies were first detected in serum samples 14 days after the first vaccination in both trials, and peaked on days 7 and 9 after the booster in the first and second trials, respectively. Low titers of neutralizing antibodies were detected in serum from only 3/6 and 2/4 animals in the first and second trial, respectively, at 14 days after the first vaccination. The titers increased 2 to 3-fold after the booster vaccination. SBV-specific RNA was detected in the serum and selective tissues in all animals after SBV challenge independent of vaccination status. The SBV candidate vaccines neither prevented viremia nor conferred protection against SBV infection.

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ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

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