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논문 기본 정보

자료유형
학술저널
저자정보
Dong-Kun Yang (Ministry of Agriculture, Food and Rural Affairs) Ha-Hyun Kim (Ministry of Agriculture, Food and Rural Affairs) Soon-Seek Yoon (Ministry of Agriculture, Food and Rural Affairs) Hyunkyoung Lee (Ministry of Agriculture, Food and Rural Affairs) In-Soo Cho (Ministry of Agriculture, Food and Rural Affairs)
저널정보
대한수의학회 Korean Journal of Veterinary Research(대한수의학회지) Korean Journal of Veterinary Research(구 대한수의학회지) 제58권 제4호
발행연도
2019.2
수록면
177 - 182 (6page)

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초록· 키워드

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Canine adenovirus type 2 (CAV-2) infection results in significant respiratory illness in dogs. Isolating and culturing CAV-2 allows for investigations into its pathogenesis and the development of vaccines and diagnostic assays. In this study, we successfully isolated a virus from a naturally infected dog in Gyeonggi-do, Korea. The virus was propagated in Madin-Darby canine kidney (MDCK) and Vero cells and showed a specific cytopathic morphology that appeared similar to a bunch of grapes. The virus was first confirmed as CAV-2 based on these cytopathic effects, an immunofluorescence assay, hemagglutination assay, and electron microscopy. The viral titer of the isolate designated APQA1601 reached 106.5 50% tissue culture infections dose per mL in MDCK cells and exhibited no hemagglutination units with erythrocytes from guinea pig. The virus was also confirmed by polymerase chain reaction and next-generation sequencing. The APQA1601 strain had the highest similarity (~99.9%) with the Toronto A26/61 strain, which was isolated in Canada in 1976 when the nucleotide sequences of the full genome of the APQA1601 strain were compared with those of other CAV strains. Isolating CAV-2 will help elucidate the biological properties of CAV-2 circulating in Korean dogs.

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Abstract
Introduction
Materials and Methods
Results
Discussion
References

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