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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.12
- 수록면
- 282 - 293 (12page)
- DOI
- 10.1186/s40824-016-0077-z
이용수
초록· 키워드
오류제보하기
Background: Dityrosine crosslinking in proteins is a bioinspired method of forming hydrogels. This study compares oxidative enzyme initiators for their relative crosslinking efficiency and cytocompatibility using the same phenol group and the same material platform. Four common enzyme and enzyme-like oxidative initiators were probed for resulting material properties and cell viability post-encapsulation. Results: All four initiators can be used to form phenol-crosslinked hydrogels, however gelation rates are dependent on enzyme type, concentration, and the oxidant. Horseradish peroxidase (HRP) or hematin with hydrogen peroxide led to a more rapid poly (vinyl alcohol)-tyramine (PVA-Tyr) polymerization (10?60 min) because a high oxidant concentration was dissolved within the macromer solution at the onset of crosslinking, whereas laccase and tyrosinase require oxygen diffusion to crosslink phenol residues and therefore took longer to gel (2.5+ hours). The use of hydrogen peroxide as an oxidant reduced cell viability immediately post-encapsulation. Laccase- and tyrosinase-mediated encapsulation of cells resulted in higher cell viability immediately post-encapsulation and significantly higher cell proliferation after one week of culture. Conclusions: Overall this study demonstrates that HRP/H2O2, hematin/H2O2, laccase, and tyrosinase can create injectable, in situ phenol-crosslinked hydrogels, however oxidant type and concentration are critical parameters to assess when phenol crosslinking hydrogels for cell-based applications. Abbreviations: |G*|, (Complex) dynamic shear modulus; ANOVA, Analysis of variance; DCC, 1,3-dicyclohexylcarbodiimide; DMEM, Dulbecco’s modified eagle’s medium; DMSO, Dimethyl sulfoxide; DTT, Dithiothreitol; EDTA, Ethylenediaminetetraacetic acid; FBS, Fetal bovine serum; H2O2, Hydrogen peroxide; HRP, Horseradish peroxidase; Md, Dried sample mass; MES, 2-[N-morpholino]ethanesulfonic acid; Mi, Initial wet mass; Mi,d, Initial macromer fraction; Ms, Swollen sample mass; NHS, N-hydroxysuccinimide; PBS, Dulbecco’s phosphate buffered saline; PEG, Poly(ethylene glycol); PVA, Poly(vinyl alcohol); PVA-Tyr, Poly(vinyl alcohol)-tyramine; Q, Mass swelling ratio; ROS, Reactive oxygen species; SDS, Sodium dodecyl sulfate
목차
등록된 정보가 없습니다.
참고문헌 (37)
참고문헌 신청
Hassan CM / 2000 / Cellular PVA hydrogels produced by freeze/thawing / J Appl Polym Sci 76:2075~2079
Kuo CK / 2001 / Ionically crosslinked alginate hydrogels as scaffolds for tissue engineering: part 1. Structure, gelation rate and mechanical properties / Biomaterials 22:511~521
Phelps EA / 2012 / Maleimide cross-linked bioactive PEG hydrogel exhibits improved reaction kinetics and cross-linking for cell encapsulation and in situ delivery / Adv Mater 24:64~70
Alves MH / 2012 / Degradable, click poly(vinyl alcohol)hydrogels : characterization of degradation and cellular compatibility / Biomed Mater 7:024106
McKinnon DD / 2014 / Biophysically defined and cytocompatible covalently adaptable networks as viscoelastic 3D cell culture systems / Adv Mater 26:865~872
Kuo CK / 2001 / Ionically crosslinked alginate hydrogels as scaffolds for tissue engineering: part 1. Structure, gelation rate and mechanical properties / Biomaterials 22:511~521
Phelps EA / 2012 / Maleimide cross-linked bioactive PEG hydrogel exhibits improved reaction kinetics and cross-linking for cell encapsulation and in situ delivery / Adv Mater 24:64~70
Alves MH / 2012 / Degradable, click poly(vinyl alcohol)hydrogels : characterization of degradation and cellular compatibility / Biomed Mater 7:024106
McKinnon DD / 2014 / Biophysically defined and cytocompatible covalently adaptable networks as viscoelastic 3D cell culture systems / Adv Mater 26:865~872
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