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논문 기본 정보

자료유형
학술저널
저자정보
김연규 (한국항공우주연구원) 박설현 (조선대학교) 이주희 (한국항공우주연구원) 최기혁 (한국항공우주연구원)
저널정보
한국우주과학회 Journal of Astronomy and Space Sciences Journal of Astronomy and Space Sciences 제32권 제1호
발행연도
2015.3
수록면
81 - 89 (9page)
DOI
http://dx.doi.org/10.5140/JASS.2015.32.1.81

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In this paper, we describe the development of a bioreactor for a cell-culture experiment on the International SpaceStation (ISS). The bioreactor is an experimental device for culturing mouse muscle cells in a microgravity environment. The purpose of the experiment was to assess the impact of microgravity on the muscles to address the possibility of longtermhuman residence in space. After investigation of previously developed bioreactors, and analysis of the requirementsfor microgravity cell culture experiments, a bioreactor design is herein proposed that is able to automatically culture 32samples simultaneously. This reactor design is capable of automatic control of temperature, humidity, and culture-mediuminjection rate; and satisfies the interface requirements of the ISS. Since bioreactors are vulnerable to cell contamination,the medium-circulation modules were designed to be a completely replaceable, in order to reuse the bioreactor after eachexperiment. The bioreactor control system is designed to circulate culture media to 32 culture chambers at a maximumspeed of 1 ml/min, to maintain the temperature of the reactor at 36±1°C, and to keep the relative humidity of the reactorabove 70%. Because bubbles in the culture media negatively affect cell culture, a de-bubbler unit was provided to eliminatesuch bubbles. A working model of the reactor was built according to the new design, to verify its performance, and was usedto perform a cell culture experiment that confirmed the feasibility of this device.

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