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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 487 - 497 (11page)
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초록· 키워드
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Fetal Bovine Serum (FBS) is an essential substance added to animal cell culture medium. However, its composition is unclear causing problems such as development of an immune response when cultured cells are transplanted into the human body. In this study, silk sericin, silk fibroin, and hemolymph obtained from silkworms were added to the cell culture medium in order to determine if it can replace FBS. After establishment of the cell culture, cell proliferation and expression levels of cell growth-related genes were compared with those of control cells (cells cultured in the medium with 10% FBS). Results showed that the test group treated with silk fibroin extracted from a Korean silkworm variety, Kumokjam could replace 10% FBS. In addition, expression levels of cell growth related genes such as Fibronectin and TGF-β1 increased significantly in cells cultured using silk fibroin, depending on the concentration used in cell adhesion and cell proliferation [24]. To date, no studies have been conducted to find a replacement for FBS. Thus, this study was carried out to develop a substitute for FBS by using silkworm-derived alternatives such as silkworm hemolymph, silk sericin, and silk fibroin, which are cheap and have various physiological effects, cell promoting effects, and can be mass produced.
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참고문헌 (42)
참고문헌 신청
Spees JL / 2004 / Internalized antigens must be removed to prepare hypoimmunogenic mesenchymal stem cells for cell and gene therapy / Mol. Ther 9:747~756
Van der Valk J / 2018 / Fetal Bovine Serum (FBS): past-present-future / ALTEX 35:99~118
Van der Valk J / 2010 / Optimization of chemically defined cell culture media – Replacing fetal bovine serum in mammalian in vitro methods / Toxicol. In Vitro 24:1053~1063
Hassan G / 2017 / simple method to isolate and expand human umbilical cord derived mesenchymal stem cells: Using explant method and umbilical cord blood serum / Int. J. Stem Cells 10:184~192
Blazquez-Prunera A / 2017 / Human mesenchymal stem cells maintain their phenotype, multipotentiality, and genetic stability when cultured using a defined xenofree human plasma fraction / Stem Cell Res. Ther 8:103~113
Van der Valk J / 2018 / Fetal Bovine Serum (FBS): past-present-future / ALTEX 35:99~118
Van der Valk J / 2010 / Optimization of chemically defined cell culture media – Replacing fetal bovine serum in mammalian in vitro methods / Toxicol. In Vitro 24:1053~1063
Hassan G / 2017 / simple method to isolate and expand human umbilical cord derived mesenchymal stem cells: Using explant method and umbilical cord blood serum / Int. J. Stem Cells 10:184~192
Blazquez-Prunera A / 2017 / Human mesenchymal stem cells maintain their phenotype, multipotentiality, and genetic stability when cultured using a defined xenofree human plasma fraction / Stem Cell Res. Ther 8:103~113
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