지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.12
- 수록면
- 493 - 496 (4page)
- DOI
- 10.4014/mbl.1609.09004
이용수
초록· 키워드
오류제보하기
Peach rosette mosaic virus (PRMV) is a plant virus that was first reported in 1933 by Peach. It can infect hosts including peach, grape, blueberry, dandelion, plum, cherry tree, and weeds. PRMV is non-reportable in Korea, but it is designated as a controlled virus requiring plant quarantine. In this study, for the rapid and specific detection of PRMV, we developed an assay using loop-mediated isothermal amplification (LAMP). Comparison between conventional polymerase chain reaction (PCR) methods (real time-PCR and nested PCR) and LAMP for the detection of PRMV revealed an equivalent level of sensitivity by all the tested methods. For the LAMP assay, outer primer sets were used to amplify a 264-bp PCR product, which was then digested using the restriction enzyme Pvu II (CAG/CTG), and the visualization of two digestion fragments (207 + 57 bp) indicated a positive reaction. The developed LAMP assay for PRMV is expected to enable the rapid monitoring of PRMV in plants.
목차
등록된 정보가 없습니다.
참고문헌 (14)
참고문헌 신청
안영창 / 2008 / 등온 증폭법을 이용한 결핵균의 빠른 검출 시스템 개발 / 대한화학회지 52(3):273~280
Anthony Johnson AM / 2014 / Development of loop-mediated isothermal amplification and SYBR green real-time PCR methods for the detection of Citrus yellow mosaic badnavirus in citrus species / J. Virol. Methods 203:9~14
CABI / Peach rosette mosaic virus. In: Crop Protection Compendium / CAB International
Cation D. / 1933 / An infectious rosette on peach trees / Mich. Agric. Exp. Stn. Q. Bull 16:79~84
조민호 / 2013 / 칩 기반 등온증폭법을 이용한 약제 내성 포도상구균의 검출 / 대한화학회지 57(1):81~87
Anthony Johnson AM / 2014 / Development of loop-mediated isothermal amplification and SYBR green real-time PCR methods for the detection of Citrus yellow mosaic badnavirus in citrus species / J. Virol. Methods 203:9~14
CABI / Peach rosette mosaic virus. In: Crop Protection Compendium / CAB International
Cation D. / 1933 / An infectious rosette on peach trees / Mich. Agric. Exp. Stn. Q. Bull 16:79~84
조민호 / 2013 / 칩 기반 등온증폭법을 이용한 약제 내성 포도상구균의 검출 / 대한화학회지 57(1):81~87
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