지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.4
- 수록면
- 170 - 178 (9page)
이용수
초록· 키워드
오류제보하기
The Lily mottle virus (LMoV) impedes the growth and quality of lily crops in Lanzhou, China. Recently Arabis mosaic virus (ArMV) has been detected in LMoVinfected plants in this region, causing plant stunting as well as severe foliar symptoms, and likely posing a threat to lily production. Consequently, there is a need to develop simple, sensitive, and reliable detection methods for these two viruses to prevent them from spreading. Reverse transcription (RT) loop-mediated isothermal amplification (LAMP) assays have been developed to detect LMoV and ArMV using two primer pairs that match six conserved sequences of LMoV and ArMV coat proteins, respectively. RT-LAMP assay results were visually assessed in reaction tubes using green fluorescence and gel electrophoresis. Our assays successfully detected both LMoV and ArMV in lily plants without the occurrence of viral cross-reactivity from other lily viruses. Optimal conditions for LAMP reactions were 65°C and 60°C for 60 min for LMoV and ArMV, respectively. Detection sensitivity for both RT-LAMP assays was a hundredfold greater than that of our comparative RT-polymerase chain reaction assays. We have also found this relatively rapid, target specific and sensitive method can also be used for samples collected in the field and may be especially useful in regions with limited or no laboratory facilities.
목차
Materials and Methods
Results
Discussion
References
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Development of a One-Step Multiplex Reverse Transcription- Quantitative Polymerase Chain Reaction Assay for the Simultaneous Detection of Four Lily Viruses
Research in Plant Disease(식물병연구)
2024 .12
-
Development of a Rapid Detection Method for Potato virus X by Reverse Transcription Loop-Mediated Isothermal Amplification
The Plant Pathology Journal
2015 .09
-
Rapid and Sensitive Detection of Lettuce Necrotic Yellows Virus and Cucumber Mosaic Virus Infecting Lettuce (Lactuca sativa L.) by Reverse Transcription Loop-Mediated Isothermal Amplification
The Plant Pathology Journal
2020 .02
-
콩황화모틀모자이크바이러스의 신속검출을 위한 역전사 등온증폭법
Research in Plant Disease(식물병연구)
2016 .09
-
Reverse transcription Loop-mediated isothermal amplification을 이용한 Soybean mosaic virus의 진단
Research in Plant Disease(식물병연구)
2015 .12
-
A Reliable Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Detecting Apple stem grooving virus in Pear
Research in Plant Disease(식물병연구)
2022 .06
-
Development of a Multiplex Reverse Transcription-Polymerase Chain Reaction Assay for the Simultaneous Detection of Three Viruses in Leguminous Plants
Research in Plant Disease(식물병연구)
2018 .12
-
Nanopore Metagenomics Sequencing for Rapid Diagnosis and Characterization of Lily Viruses
The Plant Pathology Journal
2022 .10
-
Development of a Multiplex Polymerase Chain Reaction Assay for Detecting Five Previously Unreported Papaya Viruses for Quarantine Purposes in Korea
Research in Plant Disease(식물병연구)
2024 .09
-
Simple, Rapid and Sensitive Portable Molecular Diagnosis of SFTS Virus Using Reverse Transcriptional Loop-Mediated Isothermal Amplification (RT-LAMP)
Journal of Microbiology and Biotechnology
2018 .01
-
Rapid and Visual Detection of Barley Yellow Dwarf Virus by Reverse Transcription Recombinase Polymerase Amplification with Lateral Flow Strips
The Plant Pathology Journal
2022 .04
-
Application of Rapid and Reliable Detection of Cymbidium Mosaic Virus by Reverse Transcription Recombinase Polymerase Amplification Combined with Lateral Flow Immunoassay
The Plant Pathology Journal
2022 .12
-
Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid and Sensitive Detection of Barley Yellow Dwarf Virus in Oat
The Plant Pathology Journal
2020 .10
-
An Inexpensive System for Rapid and Accurate On-site Detection of Garlic-Infected Viruses by Agarose Gel Electrophoresis Followed by Array Assay
The Plant Pathology Journal
2024 .02
-
Development of Recombinase Polymerase Amplification Combined with Lateral Flow Strips for Rapid Detection of Cowpea Mild Mottle Virus
The Plant Pathology Journal
2023 .10
-
Development of a Multiplex RT-PCR for the Simultaneous Detection of Three Viruses in Cherry Plants
Research in Plant Disease(식물병연구)
2018 .09
-
Simultaneous Detection of Four Foodborne Viruses in Food Samples Using a One-Step Multiplex Reverse Transcription PCR
Journal of Microbiology and Biotechnology
2018 .01
-
Detection of Plant Pathogenic Viruses in Commercial Gochujang (Fermented Red Pepper Paste) from Korea
The Plant Pathology Journal
2020 .10
-
Rapid and Specific Detection of Apple stem grooving virus by Reverse Transcription-recombinase Polymerase Amplification
The Plant Pathology Journal
2018 .12
-
Incidence of Viral Diseases and Occurrence of Three Unreported Viruses in Yams in Korea
Research in Plant Disease(식물병연구)
2017 .03
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0102-2023-481-001730324