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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.4
- 수록면
- 318 - 326 (9page)
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We previously reported that the SbROMT3syn recombinant protein catalyzes the production of the methylated resveratrol derivatives pinostilbene and pterostilbene by methylating substrate resveratrol in recombinant E. coli. To further study the production of stilbene compounds in E. coli by the expression of enzymes involved in stilbene biosynthesis, we isolated three stilbene synthase (STS) genes from rhubarb, peanut, and grape as well as two resveratrol Omethyltransferase (ROMT) genes from grape and sorghum.
The ability of RpSTS to produce resveratrol in recombinant E. coli was compared with other AhSTS and VrSTS genes.
Out of three STS, only AhSTS was able to produce resveratrol from p-coumaric acid. Thus, to improve the solubility of RpSTS, VrROMT, and SbROMT3 in E. coli, we synthesized the RpSTS, VrROMT and SbROMT3 genes following codonoptimization and expressed one or both genes together with the cinnamate/4-coumarate:coenzyme A ligase (CCL) gene from Streptomyces coelicolor. Our HPLC and LC-MS analyses showed that recombinant E. coli expressing both ScCCL and RpSTSsyn led to the production of resveratrol when p-coumaric acid was used as the precursor. In addition, incorporation of SbROMT3syn in recombinant E. coli cells produced resveratrol and its mono-methylated derivative, pinostilbene, as the major products from p-coumaric acid. However, very small amounts of pterostilbene were only detectable in the recombinant E. coli cells expressing the ScCCL, RpSTSsyn and SbROMT3syn genes. These results suggest that RpSTSsyn exhibits an enhanced enzyme activity to produce resveratrol and SbROMT3syn cata-lyzes the methylation of resveratrol to produce pinostilbene in E. coli cells.
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참고문헌 (26)
참고문헌 신청
Baur, J. A. / 2006 / Resveratrol improves health and survival of mice on a high-calorie diet / Nature 444:337~342
Becker, J. V. / 2003 / Metabolic engineering of Saccharomyces cerevisiae for the synthesis of the wine-related antioxidant resveratrol / FEMS Yeast Res 4:79~85
Beekwilder, J. / 2006 / Production of resveratrol in recombinant microorganisms / Appl. Environ. Microbiol 72:5670~5672
Choi, O. / 2011 / Biosynthesis of plant-specific phenylpropanoids by construction of an artificial biosynthetic pathway in Escherichia coli / J. Ind. Microbiol. Biotechnol 38:1657~1665
Delaunois, B. / 2009 / Molecular engineering of resveratrol in plants / Plant Biotechnol 7:2~12
Becker, J. V. / 2003 / Metabolic engineering of Saccharomyces cerevisiae for the synthesis of the wine-related antioxidant resveratrol / FEMS Yeast Res 4:79~85
Beekwilder, J. / 2006 / Production of resveratrol in recombinant microorganisms / Appl. Environ. Microbiol 72:5670~5672
Choi, O. / 2011 / Biosynthesis of plant-specific phenylpropanoids by construction of an artificial biosynthetic pathway in Escherichia coli / J. Ind. Microbiol. Biotechnol 38:1657~1665
Delaunois, B. / 2009 / Molecular engineering of resveratrol in plants / Plant Biotechnol 7:2~12
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