지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 27 - 35 (9page)
이용수
초록· 키워드
오류제보하기
Enterotoxigenic Escherichia coli (ETEC) causes diarrhea in pigs, referred to as colibacillosis. The aim of this study was to optimize multiplex polymerase chain reaction (PCR) and immunohistochemistry (IHC) analyses of paraffin-embedded material to detect pathogenic E. coli strains causing colibacillosis in pigs. Multiplex PCR was optimized for fimbriae (F18, F4, F6, F5, and F41) and toxins (types A and B heat-stable toxins [STaP and STb], heat-labile toxin [LT], and type 2 Shiga toxin [STx<sub>2e</sub>]), and IHC was optimized for an anti-E. coli polyclonal antibody. Samples (132) from pigs received between 2006 and 2014 with clinical and histopathological diagnoses of colibacillosis were analyzed. E. coli was detected by IHC in 78.7%, and at least one virulence factor gene was detected in 71.2%. Pathogenic strains of ETEC with at least one fimbria and one toxin were detected in 40% of the samples in multiplex PCR. The most frequent virulence types were F18-STaP (7.5%), F18-STaP-STb (5.7%), and F4-STaP (3.8%). A statistically significant association was noted between virulence factors F4, F18, STaP, and STb and positive immunostaining results. Colibacillosis diagnosis through multiplex PCR and IHC of paraffin-embedded tissues is a practical approach, as samples can be fixed and stored for long periods before analysis.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Prevalence of O-serogroups, virulence genes, and F18 antigenic variants in Escherichia coli isolated from weaned piglets with diarrhea in Korea during 2008–2016
Journal of Veterinary Science
2019 .01
-
Community-acquired Escherichia coli Enteritis in Korean Children: The Clinical Application of a Stool Polymerase Chain Reaction Assay
Infection and Chemotherapy
2017 .01
-
O-serogroups, virulence genes of pathogenic Escherichia coli from diarrhoeic piglets in Korea
한국실험동물학회 학술발표대회 논문집
2016 .08
-
Virulence-associated genes in Escherichia coli isolated from diarrheic piglets in Korea
예방수의학회지
2024 .09
-
Multiplex PCR analysis of virulence genes and their influence on antibiotic resistance in Enterococcus spp. isolated from broiler chicken
Journal of Veterinary Science
2019 .05
-
자돈 분변 유래 병원성 대장균의 병원성 인자 및 항생제 내성 양상
한국가축위생학회지
2023 .03
-
Virulence factors, antimicrobial resistance patterns, and genetic characteristics of hydrogen sulfide-producing Escherichia coli isolated from swine
Korean Journal of Veterinary Research(대한수의학회지)
2015 .09
-
사료 첨가 항생제 금지 전후 돼지 설사증 유래 대장균의 병원성 인자 및 항생제 내성 유전자
Korean Journal of Veterinary Research(대한수의학회지)
2020 .09
-
Clonal and Virulence Distribution of Uropathogenic Escherichia coli Isolated from Children in Korea
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2017 .03
-
High Quality Tissue Miniarray Technique Using a Conventional TV/Radio Telescopic Antenna
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
Evaluation of Multiplex Polymerase Chain Reaction Assay for the Simultaneous Detection of Sexually Transmitted Infections Using Swab Specimen
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2020 .03
-
IRB review points for studies utilizing paraffin blocks archived in the pathology laboratory
영남의대학술지
2018 .01
-
Phenotypic and genotypic antimicrobial resistance and virulence genes of isolated from pet dogs and cats
Journal of Veterinary Science
2017 .09
-
What is your diagnosis?
WITH CAP(COMPANION ANIMAL PRACTICE)
2021 .03
-
What is your diagnosis?
WITH CAP(COMPANION ANIMAL PRACTICE)
2021 .12
-
What is your diagnosis?
WITH CAP(COMPANION ANIMAL PRACTICE)
2021 .06
-
What is your diagnosis?
WITH CAP(COMPANION ANIMAL PRACTICE)
2020 .11
-
What is your diagnosis?
WITH CAP(COMPANION ANIMAL PRACTICE)
2021 .10
-
What is your diagnosis?
WITH CAP(COMPANION ANIMAL PRACTICE)
2021 .04
-
What is your diagnosis?
WITH CAP(COMPANION ANIMAL PRACTICE)
2020 .09
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2023-528-001366408