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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.12
- 수록면
- 569 - 576 (8page)
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Bovine embryos (day 5) were cultured to day 10 with or without 100 ng/mL PGF2 in medium supplemented with control; 100 nM Dex; 1,000 U/mL recombinant human leukemia inhibitory factor (rhLIF); or Dex+rhLIF. Although the rates to development to the blastocyst were not significantly different among groups, the hatching rate after additional culture with Dex +/or rhLIF was significantly higher in all supplemented groups than the control (p ＜ 0.05). In the presence of PGF2, the hatching rate was significantly restored in all supplemented groups relative to the group treated with only PGF2 and the control (p ＜ 0.05). Embryo transfer (ET) was performed with blastocysts (day 7). PGF2 levels of control recipient cows were significantly higher in the circulatory blood samples collected 60 min after ET than in samples collected 60 min before ET (p ＜ 0.005), and were decreased in cows injected with loading medium supplemented with Dex+rhLIF (p ＜ 0.005). Pregnancy rate was significantly higher in the ET group that received supplemented embryo-loading medium than in the non-supplemented control (p ＜ 0.05). The intrauterine administration of Dex and rhLIF at ET prevented increased PGF2 in circulatory blood and resulted in enhanced pregnancy rate.
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