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지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.8
- 수록면
- 527 - 536 (10page)
- DOI
- 10.1007/s11816-021-00695-0
이용수
초록· 키워드
오류제보하기
The genetic basis of metabolic pathways that operate during fruit ripening needs to be understood before the nutritional value of the banana can be improved. The banana is a typical starch conversion fruit, and β-amylase is a key enzyme that may play an important role in starch degradation during the ripening process. Musa acuminata β-amylase 9b (MaBAM9b) is closely related to starch degradation. However, its exact function in starch degradation has not been demonstrated in banana. Stable genetic transformation to identify gene function is a time- and energy-consuming process. Thus, an efficient and rapid method is needed for functional identification. Virus-induced gene silencing (VIGS) is a reverse-genetics method based on RNA-mediated antiviral plant defense that has been used to rapidly identify gene functions in plants. The aim of this study was to optimize a transient VIGS system and functionally elucidate MaBAM9b in postharvest banana fruit. Using 2- to 4-mm-thick fruit slices, vacuum infiltration of suspensions of Agrobacterium strains carrying TRV1 and TRV2-MaBAM9b, 0.5% iodine-potassium-iodide (I2-KI) staining for 150?s, and 1:3 TRV1:TRV2-MaBAM9b cultivation at 30?mmHg for 30?s achieved an optical density (OD) of 0.8 at 600?nm; after being incubated on Murashige and Skoog (MS) media for 5?days (d), starch degradation was efficiently suppressed during postharvest banana fruit ripening, as determined by I2-KI staining, total starch content, β-amylase activity, soluble sugar content, and endogenous MaBAM9b expression. The system described here is particularly useful for studying genes and networks involved in starch conversion in fruits, which alone would not produce a visual phenotype. This system will provide a platform for functional genomics and fruit quality improvement in banana.
목차
등록된 정보가 없습니다.
참고문헌 (20)
참고문헌 신청
Bapat VA / 2010 / Ripening of fleshy fruit: molecular insight and the role of ethylene / Biotechnol Advances 28(1):94~107
Baulcombe DC / 2004 / RNA silencing in plants / Nature 431(7006):356~363
Burch-Smith TM / 2004 / Applications and advantages of virus-induced gene silencing for gene function studies in plants / Plant J 39(5):734~746
Chen SY / 2018 / FaTT12-1, a multidrug and toxin extrusion (MATE) member involved in proanthocyanidin transport in strawberry fruit / Sci Hortic 231(2018):158~165
Chen L / 2011 / Validation of reference genes for RT-qPCR studies of gene expression in banana fruit under different experimental conditions / Planta 234(2):377~390
Baulcombe DC / 2004 / RNA silencing in plants / Nature 431(7006):356~363
Burch-Smith TM / 2004 / Applications and advantages of virus-induced gene silencing for gene function studies in plants / Plant J 39(5):734~746
Chen SY / 2018 / FaTT12-1, a multidrug and toxin extrusion (MATE) member involved in proanthocyanidin transport in strawberry fruit / Sci Hortic 231(2018):158~165
Chen L / 2011 / Validation of reference genes for RT-qPCR studies of gene expression in banana fruit under different experimental conditions / Planta 234(2):377~390
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