지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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초록· 키워드
오류제보하기
The Falcon 2 camera (Thermo Fisher Scientific Inc., USA) mounted in High Resolution Bio-TEM at Korea Basic Science Institute (KBSI) was upgraded to Falcon 3EC in August 2017 (Jeong et al., 2017). Compared with Falcon 2, Falcon 3EC has better sensitivity and higher frame rate and can be operated in electron counting mode. These changes significantly increase the data quality, thereby increasing the resolution of the 3D structure. In the benchmarking test of this machine, the structure of inhibitor-free beta-galactosidase has been solved at 2.16 Å. Here we introduce main features of the new detector, and discuss about major considerations required for its operation and data collection strategies to achieve the near atomic resolution.
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등록된 정보가 없습니다.
참고문헌 (7)
참고문헌 신청
Baker, L. A. / 2010 / Radiation Damage in Electron Cryomicroscopy / Methods Enzymol 481:371~388
D'Imprima, E. / 2019 / Protein denaturation at the air-water interface and how to prevent it / Elife 8:e42747
정형섭 / 2017 / Facility for high resolution cryo-electron microscopy of biological macromolecules at Korea Basic Science Institute / Biodesign 5(3):96~102
Kuijper, M. / 2015 / FEI’s direct electron detector developments : Embarking on a revolution in cryo-TEM / J Struct Biol 192:179~187
Li, X. / 2013 / Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM / Nat Methods 10:584~590
D'Imprima, E. / 2019 / Protein denaturation at the air-water interface and how to prevent it / Elife 8:e42747
정형섭 / 2017 / Facility for high resolution cryo-electron microscopy of biological macromolecules at Korea Basic Science Institute / Biodesign 5(3):96~102
Kuijper, M. / 2015 / FEI’s direct electron detector developments : Embarking on a revolution in cryo-TEM / J Struct Biol 192:179~187
Li, X. / 2013 / Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM / Nat Methods 10:584~590
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