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LysR-type transcriptional regulators (LTTRs) belong to the largest family of transcriptional regulators in prokaryotes.
However, the crystal structures of only a few full-length LTTRs have been determined. LTTR HypT (also known as YjiE orQseD) from Escherichia coli specifically senses hypochlorite to protect oxidation damage by hypochlorite generated bythe host immune system. In the genome of the highly virulent bacteria, Vibrio vulnificus, VV2_1132 showed the highestsequence similarity to E. coli HypT. In this study, we overexpressed full-length VV2_1132 in an E. coli expression systemand crystallized the protein. The crystal diffracted X-rays to 2.2 A resolution and belonged to the orthogonal space groupP21212, with unit cell parameters a = 57.8, b = 113.5, and c = 220.7 A. Cell content analysis predicted that the asymmetricunit may contain four molecules of VV2_1132. For the case of four molecules in the asymmetric unit, the Matthewscoefficient was 2.70 A3/Da with a solvent content of 54.5%. We are currently identifying the crystal structure of VV2_1132using anomalous signals from selenomethione-substituted crystals. This crystal structure will help elucidate the functionand action mechanism of VV2_1132, which may be involved in the pathogenesis of V. vulnificus.
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참고문헌 (16)
참고문헌 신청
Choi, H / 2001 / Structural basis of the redox switch in the OxyR transcription factor / Cell 105:103~113
Das, N / 2015 / Structure of CrgA, a cell division structural and regulatory protein from Mycobacterium tuberculosis, in lipid bilayers / Proc Natl Acad Sci USA 112:E119~E126
Drazic, A / 2014 / Tetramers are the activation-competent species of the HOCl-specific transcription factor HypT / J Biol Chem 289:977~986
Drazic, A / 2013 / Methionine oxidation activates a transcription factor in response to oxidative stress / Proc Natl Acad Sci USA 110:9493~9498
Gebendorfer, K. M / 2012 / Identification of a hypochlorite-specific transcription factor from Escherichia coli / J Biol Chem 287:6892~6903
Das, N / 2015 / Structure of CrgA, a cell division structural and regulatory protein from Mycobacterium tuberculosis, in lipid bilayers / Proc Natl Acad Sci USA 112:E119~E126
Drazic, A / 2014 / Tetramers are the activation-competent species of the HOCl-specific transcription factor HypT / J Biol Chem 289:977~986
Drazic, A / 2013 / Methionine oxidation activates a transcription factor in response to oxidative stress / Proc Natl Acad Sci USA 110:9493~9498
Gebendorfer, K. M / 2012 / Identification of a hypochlorite-specific transcription factor from Escherichia coli / J Biol Chem 287:6892~6903
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