Staphylococcus aureus derived hyaluronic acid and bacillus Calmette-Guerin purified proteins as immune enhancers to rabies vaccine and related immuno-histopathological alterations

Rania Ibrahim Shebl; Mohamed E. Amer; Tamer M. M. Abuamara; Emadeldin R. Matar; Hassan Fathy Ahmed; Tamer Albasyoni Gomah; Laila E. El Moselhy; Mohammed Abu-Elghait; Aly Fahmy Mohamed

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저자정보: Rania Ibrahim Shebl (Ahram Canadian University) Mohamed E. Amer (Al-Azhar University) Tamer M. M. Abuamara (Al-Azhar University) Emadeldin R. Matar (Al-Azhar University) Hassan Fathy Ahmed (Al-Azhar University) Tamer Albasyoni Gomah (Al-Azhar University) Laila E. El Moselhy (Al-Azhar University) Mohammed Abu-Elghait (Al-Azhar University) Aly Fahmy Mohamed (International Center for Training and Advanced Researches (ICTAR-Egypt))

저널정보: 대한백신학회 Clinical and Experimental Vaccine Research Clinical and Experimental Vaccine Research Vol.10 No.3

발행연도: 2021.9

수록면: 229 - 239 (11page)

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Purpose: One of the essential goals regarding the successful control of rabies infection is the development of a safe, effective, and inexpensive vaccine. the current study aimed to evaluate the inactivation potential of β-propiolactone (βPL), binary ethyleneimine (BEI), and hydrogen peroxide (H2O2). Materials and Methods: Estimating the inactivation kinetics of βPL, BEI, and H2O2 revealed that the tested inactivants could completely and irreversibly inactivate rabies virus within 2, 12, and 4 hours, respectively while maintaining its viral immunogenicity. The potency of βPL, BEI, and H2O2 inactivated vaccines was higher than the World Health Organization acceptance limit and were in the order of 3.75, 4.21, and 3.64 IU/mL, respectively. Monitoring the humoral and cellular immunity elicited post-immunization using Staphylococcus aureus derived hyaluronic acid (HA) and bacillus Calmette-Guerin purified protein derivative (PPD) adjuvanted rabies vaccine candidates were carried out using enzyme-linked immunosorbent assay. Results: Results demonstrated that both adjuvants could progressively enhance the release of anti-rabies total immunoglobulin G as well as the pro-inflammatory mediators (interferon-gamma and interleukin-5) relative to time. However, a higher immune response was developed in the case of HA adjuvanted rabies vaccine compared to PPD adjuvanted one. The harmful consequences of the tested adjuvants were considered via investigating the histopathological changes in the tissues of the immunized rats using hematoxylin and eosin stain. Lower adverse effects were observed post-vaccination with HA and PPD adjuvanted vaccines compared to that detected following administration of the currently used alum as standard adjuvant. Conclusion: Our findings suggested that HA and PPD could serve as a promising platform for the development of newly adjuvanted rabies vaccines with elevated immune enhancing potentials and lower risk of health hazards.

#β-Propiolactone #Binary ethyleneimine #Immune enhancers #Hyaluronic acid #Rabies vaccines

참고문헌 (34)

참고문헌 신청

Masiira B / 2018 / Long term trends and spatial distribution of animal bite injuries and deaths due to human rabies infection in Uganda, 2001-2015 / PLoS One 13:e0198568

O’Sullivan B / 2019 / Notes from the field: rabies exposures from fox bites and challenges to completing postexposure prophylaxis after Hurricane Irma: Palm Beach County, Florida, August-September 2017 / MMWR Morb Mortal Wkly Rep 68:795~797

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