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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한백신학회 Clinical and Experimental Vaccine Research Clinical and Experimental Vaccine Research Vol.10 No.3
- 발행연도
- 2021.9
- 수록면
- 217 - 229 (13page)
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초록· 키워드
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Purpose: Leptospirosis caused by Leptospira spp. remains a global health problem. Available commercial leptospiral vaccines have shown an ineffective prevention for leptospiral infection. The aim of this study was to develop leptospirosis vaccine using recombinant attenuated Salmonella vaccine (RASV) as a platform. We expected that this vaccine has ability to continuous and strongly stimulate immune systems including protective mucosal, humoral, and cell mediated immunity in rat model.
Materials and Methods: In this study, we engineered RASV, NRSL32 strain containing chromosomal fusion between nucleotides encoding secretion signal of SPI-2 effector protein, SspH2 and gene encoding major pathogenic leptospiral outer membrane lipoprotein, LipL32. Subsequently, our modified RASV was oral vaccination to rat and blood samples were taken for assessment of immune responses.
Results: Our Salmonella NRSL32 strain showed expression and secretion of SspH21-215-LipL32 recombinant protein via SPI-2 T3SS. After oral administration of NRSL32 strain to rats, significant titers of total immunoglobulin G (IgG) and immunoglobulin A against rLipL32 were observed in long period up to 77 days after vaccination. The stimulated antibody showed ability to specific bind with LipL32 protein on surface of pathogenic Leptospira spp. Additionally, the balance level of IgG2a/IgG1 ratio and level of interferon-γ and interleukin-4 secretion were detected.
Conclusion: The results showed that our RASV platform with chromosomal expression elicited effective immune responses to leptospiral antigen. Moreover, this platform was capable for simultaneous stimulation of Th1 and Th2-biased responses. Further investigation is necessary study of protective efficacy against leptospiral infection in animal models.
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참고문헌 (39)
참고문헌 신청
Sun AH / 2020 / Leptospirosis is an invasive infectious and systemic inflammatory disease / Biomed J 43:24~31
Munoz-Zanzi C / 2020 / A systematic literature review of leptospirosis outbreaks worldwide, 1970-2012 / Rev Panam Salud Publica 44:e78
Seguro AC / 2013 / Pathophysiology of leptospirosis / Shock 39(Suppl 1):17~23
Haake DA / 2015 / Leptospirosis in humans / Curr Top Microbiol Immunol 387:65~97
Samir A / 2015 / Leptospirosis in animals and human contacts in Egypt : broad range surveillance / Rev Soc Bras Med Trop 48:272~277
Munoz-Zanzi C / 2020 / A systematic literature review of leptospirosis outbreaks worldwide, 1970-2012 / Rev Panam Salud Publica 44:e78
Seguro AC / 2013 / Pathophysiology of leptospirosis / Shock 39(Suppl 1):17~23
Haake DA / 2015 / Leptospirosis in humans / Curr Top Microbiol Immunol 387:65~97
Samir A / 2015 / Leptospirosis in animals and human contacts in Egypt : broad range surveillance / Rev Soc Bras Med Trop 48:272~277
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