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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 충북대학교 동물의학연구소 Journal of Biomedical and Translational Research Journal of Biomedical and Translational Research 제19권 제3호
- 발행연도
- 2018.9
- 수록면
- 43 - 48 (6page)
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This study was carried out to investigate the protectiveeffect of prednisolone in rabbitprimary cultured articular chondrocytes treated with sodiumnitroprusside (SNP), a nitric oxide donor. After cell phenotype was determined, the MTT assay and Western blot analysis of type II collagen, cylooxygenase-2 (COX-2) and phosphorylatedextracellular regulated kinase (pERK) were performed in control, SNP(298μg/ml) alone orSNP plus prednisolone (0.05-50 μg/ml)-treated rabbit articular chondrocytes. Immunofluorescence staining of type II collagen was also performed. Cell morphology indicated that SNP treatment induced cytotoxicity, and that SNP-induced cytotoxicity was inhibited by prednisolone treatment. MTT assay showed thatSNP treatment decreasedsignificantly the level of cell viability comparedwith that of control (p<0.01), and that prednisolonetreatment decreased SNP-induced cytotoxicity.SNPtreatment decreased type II collagen compared with control chondrocytes, and prednisolone treatment recovered the down-regulatedexpression of type IIcollagen inducedby SNP, showing a significant level in 5 μg/ml of the prednisolone treatment group compared to the SNPtreatment group (p < 0.05). Increase of COX-2 was significantly induced by SNP treatment compared to control chondrocytes (p<0.01), and COX-2 expression was decreased by prednisolone treatment, showing a significant level in 50 μg/ml of theprednisolone treatment group compared to theSNP treatment group (p < 0.05). Immunofluorescence staining confirmed these phenomena. Furthermore, SNP treatment significantly induced a decrease of pERK expression compared tocontrol chondrocytes (p<0.01), and prednisolonetreatment recovered its expression, showing a significant level in 0.5 μg/ml of the prednisolone treatment group compared to the SNPtreatment group (p < 0.05). Taken together, prednisolone inhibited SNP-induced celldeath and dedifferentiation, and modulated expression of COX-2 and pERK inrabbit articular chondrocytes.
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참고문헌 (21)
참고문헌 신청
Sandell LJ / 2001 / Articular cartilage and changes in arthritis. An introduction: cell biology of osteoarthritis / Arthritis Res 3:107~113
Blanco FJ / 1998 / Osteoarthritis chondrocytes die by apoptosis. A possible pathway for osteoarthritis pathology / Arthritis Rheum 41:284~289
Hashimoto S / 1998 / Linkage of chondrocyte apoptosis and cartilage degradation in human osteoarthritis / Arthritis Rheum 41:1632~1638
Otero M / 2007 / Cells of the synovium in rheumatoid arthritis. Chondrocytes / Arthritis Res Ther 9:220
Mates JM / 1999 / Antioxidant enzymes and human diseases / Clin Biochem 32:595~603
Blanco FJ / 1998 / Osteoarthritis chondrocytes die by apoptosis. A possible pathway for osteoarthritis pathology / Arthritis Rheum 41:284~289
Hashimoto S / 1998 / Linkage of chondrocyte apoptosis and cartilage degradation in human osteoarthritis / Arthritis Rheum 41:1632~1638
Otero M / 2007 / Cells of the synovium in rheumatoid arthritis. Chondrocytes / Arthritis Res Ther 9:220
Mates JM / 1999 / Antioxidant enzymes and human diseases / Clin Biochem 32:595~603
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