지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 충북대학교 동물의학연구소 Journal of Biomedical and Translational Research Journal of Biomedical and Translational Research 제18권 제2호
- 발행연도
- 2017.6
- 수록면
- 56 - 59 (4page)
이용수
초록· 키워드
오류제보하기
As diethylnitrosamine (DEN) effect on cell proliferation, DNA damage and stem cell marker(s) expression have been largely unknown in mouse normal hepatocytes (AML-12 cells) cultured over a short-term period, this study was conducted to examine the cell proliferation, Ataxia telangiectasia mutated (ATM) and epithelial cell adhesion molecule (EpCAM) and Neighbor of Punc E 11 (Nope) expression in AML-12 cells treated with DEN for 24 and 48 h. Cells were treated with DEN (25-800 μg/mL) and cell phenotype was determined, and the MTT assay was used to quantify the proliferation of cells treated with DEN. Expression and distribution of ATM in AML-12 cells were determined by indirect immunofluorescence microscopy. And Western blot analysis of EpCAM and Nope was performed. Cell viability was significantly increased in response to all doses of DEN treatment compared to control at 24 h (p<0.05 or p<0.01). However, there was no significant increase at 48 h, even though it showed increased trend. Immunofluorescence staining of ATM showed that there was an increase of ATM expression at doses of 50, 100 and 200 μg/mL of DEN treatment, showing strong nuclear staining. Furthermore, Western blot analysis showed that DEN treatment showed increased trend of EpCAM and Nope expression. Taken together, DEN treatment increased cell proliferation in AML-12 cells, and it was associated with increased ATM expression.
목차
등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
Kenyon J / 2007 / The role of DNA damage repair in aging of adult stem cells / Nucleic Acids Res 35(22):7557~7565
Wicha MS / 2006 / Cancer stem cells : an old idea--a paradigm shift / Cancer Res 66(4):1883~1890
Visvader JE / 2008 / Cancer stem cells in solid tumours : accumulating evidence and unresolved questions / Nat Rev Cancer 8(10):755~768
Alison MR / 2005 / Liver stem cells : implications for hepatocarcinogenesis / Stem Cell Rev 1(3):253~260
Vesselinovitch SD / 1984 / Carcinogenicity of diethylnitrosamine in newborn, infant, and adult mice / J Cancer Res Clin Oncol 108(1):60~65
Wicha MS / 2006 / Cancer stem cells : an old idea--a paradigm shift / Cancer Res 66(4):1883~1890
Visvader JE / 2008 / Cancer stem cells in solid tumours : accumulating evidence and unresolved questions / Nat Rev Cancer 8(10):755~768
Alison MR / 2005 / Liver stem cells : implications for hepatocarcinogenesis / Stem Cell Rev 1(3):253~260
Vesselinovitch SD / 1984 / Carcinogenicity of diethylnitrosamine in newborn, infant, and adult mice / J Cancer Res Clin Oncol 108(1):60~65
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Altered expression of epithelial cell adhesion molecule in hepatocytes treated with diethynitrosamine
한국실험동물학회 학술발표대회 논문집
2015 .08
-
Clonal Expansion of Allergen-specific CD4+ T Cell in the Lung in the Absence of Lymph Nodes
Immune Network
2017 .06
-
Immune Correlates of Resistance to Trichinella spiralis Reinfection in Mice
Parasites, Hosts and Diseases
2016 .10
-
PBMC cells Telomere length change depending on the diethylnitrosamine(DEN) Concentrations in mouse
한국실험동물학회 학술발표대회 논문집
2017 .08
-
Induction of cell deathin early developmental phase of zebrafish treated with diethylnitrosamine
Journal of Biomedical and Translational Research
2019 .01
-
Exosomes Secreted by Toxoplasma gondii-Infected L6 Cells : Their Effects on Host Cell Proliferation and Cell Cycle Changes
Parasites, Hosts and Diseases
2016 .04
-
Heterogeneity of Islet Cells during Embryogenesis and Differentiation
Diabetes and Metabolism Journal
2023 .03
-
Regulation of Th2 Cell Immunity by Dendritic Cells
Immune Network
2016 .02
-
Expression of p63 and its association with cell proliferation at different stages of murine hair follicle cycle
Journal of Biomedical and Translational Research
2018 .03
-
Immunohistochemical Expression and Clinical Significance of Suggested Stem Cell Markers in Hepatocellular Carcinoma
Journal of Pathology and Translational Medicine
2016 .01
-
Self-organized insulin-producing β-cells differentiated from human omentum-derived stem cells and their in vivo therapeutic potential
생체재료학회지
2023 .03
-
Paracrine influence of human perivascular cells on the proliferation of adenocarcinoma alveolar epithelial cells
The Korean Journal of Physiology & Pharmacology
2017 .03
-
Cytotoxicity of natural killer cells co-cultured with γ-irradiatedor mitomycin C-treated feeder cells
예방수의학회지
2020 .01
-
Advanced Properties of Urine Derived Stem Cells Compared to Adipose Tissue Derived Stem Cells in Terms of Cell Proliferation, Immune Modulation and Multi Differentiation
Journal of Korean Medical Science
2015 .01
-
Regeneration of Damaged Tissues After Cell Transplantation in Cardiovascular Animal Models
한국실험동물학회 학술발표대회 논문집
2015 .08
-
Diethylnitrosamine(DEN) - induced epigenetic damage (COX-2 gene) in three organs (liver, spleen, colon) in mice based on the Gene methylation
한국실험동물학회 학술발표대회 논문집
2017 .08
-
Alternative Therapy Trends among Korean Postmenopausal Women
대한폐경학회지
2016 .01
-
SD-317 inhibits cell growth and proliferation by preventing activation of Akt in lung cancer
한국실험동물학회 학술발표대회 논문집
2020 .07
-
Expression of Estrogen Receptor-alpha in Nasal Polyps and the Effects of Dexamethasone on Estrogen Receptor-alpha Expression in RPMI 2650 Cells
Journal of Korean Medical Science
2020 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0