지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.12
- 수록면
- 227 - 237 (11page)
- DOI
- 10.7853/kjvs.2021.44.4.227
이용수
초록· 키워드
오류제보하기
Artificial insemination of Korean native cattle (KNC) is the predominant method for breed improve-ment. However, industrialization of embryo production and transfer is necessary to utilize the ge-netic potential of KNC. The aim of this study was to examine associations between KNC donor cows and ovum pick-up (OPU) conditions, in-vivo oocyte recovery, and embryo development. Oocyte recovery and blastocyst development rates were higher at 50 and 60 mmHg OPU vacuum pressure than at 40 mmHg, which was, however, not significant. Regarding follicle growth, injection of 500 mg GnRH 36 hours before OPU significantly increased the number of OPU oocytes from an average of 4.6 to 7.6 (P <0.05); no significant difference in embryo development rates was observed. Significant differences were observed in the numbers of OPU oocytes, embryo development rates, and trans-plantable blastocysts per individual among nine KNC donors (P <0.05). Furthermore, although there was no difference in OPU oocyte recovery intervals in approximately 2∼8 weeks, the number of recovered oocytes significantly decreased at the 12-week interval (P <0.05); there was no difference in embryo development rates. The number of oocytes and embryonic development rates only tended to decrease until the seventh OPU session, but decreased significantly until the eighth session (P <0.05). The average pregnancy rate after transfer of OPU-derived in-vitro embryos into recipient cows was 41.8%. To improve the efficiency of OPU egg recovery and in-vitro embryo production, considering KNC donor characteristics, vacuum pressure of 60 mmHg, GnRH pretreatment to induce follicle growth, and effective OPU egg recovery up to seven times at intervals of 2∼4 weeks appears to be most suitable. This study may facilitate the industrialization of KNC embryo production and transfer using high-quality cows.
목차
등록된 정보가 없습니다.
참고문헌 (51)
참고문헌 신청
농림축산식품부 / 2020 / 정액증명서등 / 축산법 제18조
Blondin P / 1995 / Oocyte and follicular morphology as determining characteristics for developmental competence in bovine oocytes / Mol. Reprod. Dev 41:54~62
Bols PEJ / 1997 / Effect of needle tip bevel and aspiration procedure on the morphology and developmental capacity of bovine compact cumulus oocytes complexes / Theriogenology 46:899~909
Chastant-Maillard S / 2003 / Consequences of transvaginal follicular puncture on well-being in cows / Reproduction 125:555~563
Chaubal SA / 2007 / Hormonal treatments for increasing the oocyte and embryo production in an OPU-IVP system / Theriogenology 67:719~728
Blondin P / 1995 / Oocyte and follicular morphology as determining characteristics for developmental competence in bovine oocytes / Mol. Reprod. Dev 41:54~62
Bols PEJ / 1997 / Effect of needle tip bevel and aspiration procedure on the morphology and developmental capacity of bovine compact cumulus oocytes complexes / Theriogenology 46:899~909
Chastant-Maillard S / 2003 / Consequences of transvaginal follicular puncture on well-being in cows / Reproduction 125:555~563
Chaubal SA / 2007 / Hormonal treatments for increasing the oocyte and embryo production in an OPU-IVP system / Theriogenology 67:719~728
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