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논문 기본 정보

자료유형
학술저널
저자정보
홍은설 (건양사이버대학교) 송연숙 (한남대학교) 이근광 (고구려대학교)
저널정보
한국미용학회 한국미용학회지 한국미용학회지 제24권 제6호
발행연도
2018.12
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1,306 - 1,315 (10page)

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The purpose was to investigate the cytopathic effect and cell viability on cultivated HaCaT cell lines after processing for 24 hours and 48 hours through treatment with differing concentrations of PPD. The results are as fellows: Normal HaCaT cell lines grew normally in a cell cultivation flask, and were almond shaped. HaCaT cell lines that were exposed to the toxicity of PPD were generally inactive and showed signs of the cytopathic effect because of weak adhesion. Less than 50% cell viability was observed after 50 minutes in a group with 0.5% PPD, About 48% cell viability was observed after 10 minutes in a group with 1~3% PPD, and more than 45~40% cell viability was observed after 10 minutes in a group with 4~5% of PPD. The results due to the degree of cytotoxicity show that; The survival rate of cultivated cells after 48 hours increased after treatment for 10 minutes for a group with 0.5~1% PPD compared to cultivated cells treated for 24 hours. This suggests cells recovered little by little after 48 hours from a group with less than 1% of the treatment concentration for 10 minutes. But, they didn’t recover in the group with 2~5% of the treatment concentration. Therefore, the survival rate of HaCaT cells went down and cell viability went up after increasing the treatment concentration and treatment time in relation to the degree of cell viability from the PPD. Cell numbers increased because of the recovery of cells after 48 hours with treatment for 10 minutes from groups with 0.5% and 1% (54.93% survival rate in 0.5% PPD, 49.25% survival rate in 1% PPD). The cytotoxicity rate decreased with the lower concentration treatment and shorter treatment time.

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