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Reactions between gold (Au) surface plasmon resonance (SPR) chips and bovine serum albumin (BSA) dissolved in solutions of different pH were investigated. The charge on the BSA depends on the pH of the solution in which it is dissolved. Thus, dissolving BSA in different pH solutions resulted in different charges of BSA. Among the BSA dissolved in solutions with pH 4.01, 7.4, and 10.01, the SPR response was the highest for BSA dissolved in the solution of pH 4.01. To eliminate the response variation owing to the difference in the refractive indices of the solutions, phosphate buffered saline (PBS) was injected into the system after the reaction of BSA with the Au SPR chip had happened. In this case too, the BSA dissolved in the solution with pH 4.01 exhibited the highest response. This may be attributed to the non-uniform distribution of ionic patches on the BSA, which can induce electrostatic attraction to the surface even though BSA has a positive charge at pH 4.01, and the absolute values of the net charge of BSA at pH 4.01 and 7.4 were very close.
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참고문헌 (18)
참고문헌 신청
H. Šípová / 2013 / Surface plasmon resonance sensing of nucleic acids : A review / Anal. Chim. Acta 773:9~23
V. Yesudasu / 2021 / Recent progress in surface plasmon resonance based sensors:A comprehensive review / Heliyon 7:e06321(1)~e06321(13)
Q. Wang / 2021 / Recent progress of surface plasmon resonance in the development of coronavirus disease-2019drug candidates / Eur. J. Med. Chem. Rep 1:100003(1)~100003(10)
P. Falkowski / 2020 / Potential of surface plasmon resonance biosensors in cancer detection / J. Pharm. Biomed. Anal 194:113802(1)~113802(11)
C. Situ / 2010 / Advances in surface plasmon resonance biosensor technology towards high-throughput, food-safety analysis / Trends. Analyt. Chem. 29(11):1305~1315
V. Yesudasu / 2021 / Recent progress in surface plasmon resonance based sensors:A comprehensive review / Heliyon 7:e06321(1)~e06321(13)
Q. Wang / 2021 / Recent progress of surface plasmon resonance in the development of coronavirus disease-2019drug candidates / Eur. J. Med. Chem. Rep 1:100003(1)~100003(10)
P. Falkowski / 2020 / Potential of surface plasmon resonance biosensors in cancer detection / J. Pharm. Biomed. Anal 194:113802(1)~113802(11)
C. Situ / 2010 / Advances in surface plasmon resonance biosensor technology towards high-throughput, food-safety analysis / Trends. Analyt. Chem. 29(11):1305~1315
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