지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.9
- 수록면
- 154 - 164 (11page)
- DOI
- 10.5010/JPB.2021.48.3.156
이용수
초록· 키워드
오류제보하기
Spirodela polyrhiza belongs to the family Lemnaceae, small aquatic plants that offer an alternative plant-based system for the expression of recombinant proteins. However, no turion transformation protocol has yet been established in this species. In this study, we exploited a pB7YWG2 vector harboring the eYFP gene that encodes enhanced yellow fluorescent protein (eYFP), which has been extensively used as a reporter marker for the visualization of the recombinant protein localization in plants. Agrobacterium tumefaciens-mediated turion transformation via vacuum infiltration was adopted to deliver the eYFP gene to turions, special vegetative forms of duckweeds produced for enduring harsh conditions. Transgenic turions were able to regenerate several duckweed fronds that exhibit yellow fluorescent emissions under a fluorescence microscope. The expression of the eYFP protein was subsequently verified via western blotting. To the best of our knowledge, this paper is the first to report an efficient protocol for generating transgenic S. polyrhiza expressing eYFP via Agrobacterium tumefaciens-mediated turion transformation. The ability of turions to withstand harsh conditions will favor the portability and versatility of transgenic duckweeds for further development of therapeutic compounds in plants.
목차
등록된 정보가 없습니다.
참고문헌 (46)
참고문헌 신청
Appenroth KJ / 2013 / Telling duckweed apart: Genotyping technologies for Lemnaceae / Chin J Appl Environ Biol 19:1~10
Áy Z / 2012 / The Effect of High Concentrations of glufosinate ammonium on the Yield Components of Transgenic Spring Wheat (Triticum aestivum L.) Constitutively Expressing the bar Gene / Sci World J 2012:657945
Boehm R / 2001 / A transient transformation system for duckweed (Wolffia columbiana) using Agrobacterium-mediated gene transfer / J Appl Bot Food Qual 75(3):107~111
Budhagatapalli N / 2016 / A simple test for the cleavage activity of customized endonucleases in plants / Plant Methods 12:18
Cantó-Pastor A / 2015 / Efficient transformation and artificial miRNA gene silencing in Lemna minor / Plant Biol (Stuttg) 17(Supplement 1):59~65
Áy Z / 2012 / The Effect of High Concentrations of glufosinate ammonium on the Yield Components of Transgenic Spring Wheat (Triticum aestivum L.) Constitutively Expressing the bar Gene / Sci World J 2012:657945
Boehm R / 2001 / A transient transformation system for duckweed (Wolffia columbiana) using Agrobacterium-mediated gene transfer / J Appl Bot Food Qual 75(3):107~111
Budhagatapalli N / 2016 / A simple test for the cleavage activity of customized endonucleases in plants / Plant Methods 12:18
Cantó-Pastor A / 2015 / Efficient transformation and artificial miRNA gene silencing in Lemna minor / Plant Biol (Stuttg) 17(Supplement 1):59~65
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