Efficient Agrobacterium-mediated in planta genetic transformation of watermelon [Citrullus lanatus Thunb.]

Vasudevan Venkatachalam; Sathish Dorairaj; Ajithan Chandrasekaran; Sathish Selvam; Manickavasagam Markandan

doi:10.1007/s11816-021-00691-4

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자료유형: 학술저널

저자정보: Vasudevan Venkatachalam (Department of Biotechnology Bharathidasan University Tiruchirappalli) Sathish Dorairaj (Department of Biotechnology Bharathidasan University Tiruchirappalli) Ajithan Chandrasekaran (Department of Biotechnology Bharathidasan University Tiruchirappalli) Sathish Selvam (Department of Biotechnology Bharathidasan University Tiruchirappalli) Manickavasagam Markandan (Department of Biotechnology Bharathidasan University Tiruchirappalli)

저널정보: 한국식물생명공학회 Plant Biotechnology Reports Plant Biotechnology Reports 제15권 제4호

발행연도: 2021.8

수록면: 447 - 457 (11page)

DOI: 10.1007/s11816-021-00691-4

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The production of transgenic watermelon through Agrobacterium tumefaciens-mediated transformation with in?vitro regen- eration system is a time-consuming, labor-intensive and genotype-dependent process. To acquire large number of transgenic watermelons in a shorter period of time, the half-seed explants were infected with Agrobacterium strain EHA 105 harboring pCAMBIA1301 with bar and gus genes. The factors affecting in planta transformation efficiency, such as co-cultivation duration, acetosyringone concentration, sonication duration and vacuum infiltration were assessed in the present study. The half-seed explants were sonicated for 3?min and 2?min vacuum infiltrated in Agrobacterium suspension and co-cultivated for 3?days with 100?μM acetosyringone showed maximum transformation efficiency. The transformed watermelon plants were selected against BASTA® and GUS, PCR, Southern hybridization analysis confirmed the transgene integration. The amenability of this established protocol was analyzed on four genotypes, in which the response of all genotypes was posi- tive, whereas Arka manik showed the higher transformation efficiency of 17%. The transformation protocol developed in the present study is efficient, economical and expeditious without the taking part of any tissue culture phases and produce a large number of transgenic lines within a short period of 56?days.

#Agrobacterium tumefaciens #In planta genetic transformation #Sonication #Vacuum infiltration #Watermelon

참고문헌 (22)

참고문헌 신청

Akashi K / 2005 / Agrobacterium-mediated transformation system the drought and excess light stresstolerant wild watermelon (Citrullus lanatus) / Plant Biotechnol 22(1):13~18

Bakshi S / 2011 / Improved Agrobacterium- mediated transformation of cowpea via sonication and vacuum infiltration / Plant Cell Rep 30(12):2281~2292

Chen X / 2010 / A high-throughput transient gene expression system for switchgrass (Panicum virgatum L.) seedlings / Biotechnol Biofuel 3(9):1~10

Cho MA / 2008 / Agrobacterium-mediated transformation in Citrullus lanatus / Biol Plant 52(2):365~369

Gaster H / 1997 / The potential role of lycopene for human health / J Amer Coll Nutr 16(2):109~126

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