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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.10
- 수록면
- 379 - 394 (16page)
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Purpose. In our previous studies, application of trichloroacetic acid (TCA) to gingival fibroblasts or to canine palatal soft tissue was verified to alter the expression of several genes responsible for cell cycle progression. In order to confirm this effect in a system allowing sequential release of TCA and epidermal growth factor (EGF), expression of various cell cycle genes following the application of the agents, using hydrophobically modified glycol chitosan (HGC)-based nano-controlled release system, was explored in this study.
Materials and methods. HGCbased nano-controlled release system was developed followed by loading TCA and EGF. The groups were defined as the control (CON); TCA-loaded nano-controlled release system (EXP1); TCA- and EGF- individually loaded nano-controlled releasesystem (EXP2). At 24- and 48 hr culture, expression of 37 cell cycle genes was analyzed in human gingival fibroblasts. Correlations and the influential genes were also analyzed.
Results. Numerous genes such as cyclins (CCNDs), cell division cycles (CDCs), cyclin-dependent kinases (CDKs), E2F transcription factors (E2Fs), extracellular signal-regulated kinases (ERKs) and other cell cycle genes were significantly up-regulated in EXP1 and EXP2. Also, cell cycle arrest genes of E2F4, E2F5, and GADD45G were up-regulated but another cell cycle arrest gene SMAD4 was down-regulated. From the multiple regression analysis, CCNA2, CDK4, and ANAPC4 were determined as the most influential factors on the expression of ERK genes.
Conclusion. Application of TCA and EGF, using the HGC-based nano-controlled sequential release system significantly up-regulated various cell cycle progression genes, leading to the possibility of regenerating oral soft tissue via application of the proposed system.
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참고문헌 (32)
참고문헌 신청
Dirks PB / 1997 / Current concepts in neuro-oncology : the cell cycle-a review / Neurosurgery 40:1000~1015
Vermeulen K / 2003 / The cell cycle : a review of regulation, deregulation and therapeutic targets in cancer / Cell Prolif 36:131~149
Zhang W / 2002 / MAPK signal pathways in the regulation of cell proliferation in mammalian cells / Cell Res 12:9~18
이경호 / 2019 / Chemical Regeneration of Wound Defects: Relevance to the Canine Palatal Mucosa and Cell Cycle Up-Regulation in Human Gingival Fibroblasts / 조직공학과 재생의학 16(6):675~684
Kwang Man Park / 2020 / Oral Soft Tissue Regeneration Using Nano Controlled System Inducing Sequential Release of Trichloroacetic Acid and Epidermal Growth Factor / 조직공학과 재생의학 17(1):91~103
Vermeulen K / 2003 / The cell cycle : a review of regulation, deregulation and therapeutic targets in cancer / Cell Prolif 36:131~149
Zhang W / 2002 / MAPK signal pathways in the regulation of cell proliferation in mammalian cells / Cell Res 12:9~18
이경호 / 2019 / Chemical Regeneration of Wound Defects: Relevance to the Canine Palatal Mucosa and Cell Cycle Up-Regulation in Human Gingival Fibroblasts / 조직공학과 재생의학 16(6):675~684
Kwang Man Park / 2020 / Oral Soft Tissue Regeneration Using Nano Controlled System Inducing Sequential Release of Trichloroacetic Acid and Epidermal Growth Factor / 조직공학과 재생의학 17(1):91~103
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