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논문 기본 정보

자료유형
학술저널
저자정보
Kwang Man Park (Kyung Hee University) Hong Jae Lee (Kyung Hee University) 구기태 (서울대학교) Heithem Ben Amara (Seoul National University School of Dentistry) Richard Leesungbok (Kyung Hee University School of Dentistry) Kwantae Noh (Kyung Hee University) Sang Cheon Lee (Kyung Hee University School of Dentistry) Suk-Won Lee (Kyung Hee University Hospital at Gangdong)
저널정보
한국조직공학과 재생의학회 조직공학과 재생의학 조직공학과 재생의학 제17권 제1호
발행연도
2020.1
수록면
91 - 103 (13page)

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BACKGROUND: The effect of nano controlled sequential release of trichloroacetic acid (TCA) and epidermal growth factor (EGF) on the oral soft tissue regeneration was determined. METHODS: Hydrophobically modified glycol chitosan (HGC) nano controlled system was developed for the sequential release of TCA and EGF, and the release pattern was identified. The HGC-based nano controlled release system was injected into the critical-sized defects created in beagles’ palatal soft tissues. The palatal impression and its scanned body was obtained on various time points post-injection, and the volumetric amount of soft tissue regeneration was compared among the three groups: CON (natural regeneration control group), EXP1 (TCA-loaded nano controlled release system group), EXP2 (TCA and EGF individually loaded nano controlled release system). DNA microarray analysis was performed and various soft tissue regeneration parameters in histopathological specimens were measured. RESULTS: TCA release was highest at Day 1 whereas EGF release was highest at Day 2 and remained high until Day 3. In the volumetric measurements of impression body scans, no significant difference in soft tissue regeneration between the three groups was shown in two-way ANOVA. However, in the one-way ANOVA at Day 14, EXP2 showed a significant increase in soft tissue regeneration compared to CON. High correlation was determined between the histopathological results of each group. DNA microarray showed up-regulation of various genes and related cell signaling pathways in EXP2 compared to CON. CONCLUSION: HGC-based nano controlled release system for sequential release of TCA and EGF can promote regeneration of oral soft tissue defects.

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