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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.9
- 수록면
- 1,126 - 1,141 (16page)
- DOI
- 10.5187/jast.2021.e93
이용수
초록· 키워드
오류제보하기
Recent evidence has shown that methionine (Met) supplementation can improve milk protein synthesis under hyperthermia (which reduces milk production). To explore the mechanism by which milk protein synthesis is affected by Met supplementation under hyperthermia, mammary alveolar (MAC-T) cells were incubated at a hyperthermic temperature of 42℃ for 6 h in media with different concentrations of Met. While the control group (CON) contained a normal amino acid concentration profile (60 μg/mL of Met), the three treatment groups were supplemented with Met at concentrations of 10 μg/mL (MET70, 70 μg/mL of Met), 20 μg/mL (MET80, 80 μg/mL of Met), and 30 μg/mL (MET90,90 μg/mL of Met). Our results show that additional Met supplementation increases the mRNA and protein levels of BCL2 (B-cell lymphoma-2, an anti-apoptosis agent), and decreases the mRNA and protein levels of BAX (Bcl-2-associated X protein, a pro-apoptosis agent), especially at an additional supplementary concentration of 20 μg/mL (group Met80). Supplementation with higher concentrations of Met decreased the mRNA levels of Caspase-3 and Caspase-9, and increased protein levels of heat shock protein (HSP70). The total protein levels of the mechanistic target of rapamycin (mTOR) and the mTOR signalling pathway-related proteins, AKT, ribosomal protein S6 kinase B1 (RPS6KB1), and ribosomal protein S6 (RPS6), increased with increasing Met supplementation, and peaked at 80 μg/mL Met (group Met80). In addition, we also found that additional Met supplementation upregulated the gene expression of αS1-casein (CSN1S1), β-casein (CSN2), and the amino acid transporter genes SLC38A2, SLC38A3 which are known to be mTOR targets. Additional Met supplementation, however, had no effect on the gene expression of κ-casein (CSN3) and solute carrier family 34 member 2 (SLC34A2). Our results suggest that additional Met supplementation with 20 μg/mL may promote the synthesis of milk proteins in bovine mammary epithelial cells under hyperthermia by inhibiting apoptosis, activating the AKTmTOR- RPS6KB1 signalling pathway, and regulating the entry of amino acids into these cells.
목차
등록된 정보가 없습니다.
참고문헌 (67)
참고문헌 신청
J.W. West / 2003 / Effects of Heat-Stress on Production in Dairy Cattle / Journal of Dairy Science 86(6):2131~2144
J. W. Fuquay / 1981 / Heat Stress as it Affects Animal Production / Journal of Animal Science 52(1):164~174
D.K. Beede / 1986 / Potential Nutritional Strategies for Intensively Managed Cattle during Thermal Stress / Journal of Animal Science 62(2):543~554
F.C. Cowley / 2015 / Immediate and residual effects of heat stress and restricted intake on milk protein and casein composition and energy metabolism / Journal of Dairy Science 98(4):2356~2368
S.T. Gao / 2017 / The effects of heat stress on protein metabolism in lactating Holstein cows / Journal of Dairy Science 100(6):5040~5049
J. W. Fuquay / 1981 / Heat Stress as it Affects Animal Production / Journal of Animal Science 52(1):164~174
D.K. Beede / 1986 / Potential Nutritional Strategies for Intensively Managed Cattle during Thermal Stress / Journal of Animal Science 62(2):543~554
F.C. Cowley / 2015 / Immediate and residual effects of heat stress and restricted intake on milk protein and casein composition and energy metabolism / Journal of Dairy Science 98(4):2356~2368
S.T. Gao / 2017 / The effects of heat stress on protein metabolism in lactating Holstein cows / Journal of Dairy Science 100(6):5040~5049
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