지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2022.5
- 수록면
- 481 - 499 (19page)
이용수
초록· 키워드
오류제보하기
This study aims to determine the effects of D-methionine (D-Met) isomer and the methionine precursor 2-hydroxy-4-methylthiobutanoic acid i (HMBi) supplementation on milk protein synthesis on immortalized bovine mammary epithelial cell (MAC-T). MAC-T cells were seeded using 10-cm dishes and cultured in Dulbecco’s modified Eagle’s medium/F12 (DMEM/F12) basic medium. The basic medium of DMEM/F12 was replaced with the lactogenic DMEM/ F12 differentiation medium when 90% of MAC-T cells reached confluency. The best dosage at 0.6 mM of D-Met and HMBi and incubation time at 72 h were used uniformly for all treatments. Each treatment was replicated six times wherein treatments were randomly assigned in a 6-well plate. Cell, medium, and total protein were determined using a bicinchoninic acid protein assay kit. Genes, proteomics and metabolomics analyses were also done to determine the mechanism of the milk protein synthesis pathway. Data were analyzed by two-way analysis of variance (ANOVA) with supplement type and plate as fixed effects. The least significant difference test was used to evaluate the differences among treatments. The HMBi treatment group had the highest beta-casein and S6 kinase beta-1 (S6K1) mRNA gene expression levels. HMBi and D-Met treatments have higher gene expressions compared to the control group. In terms of medium protein content, HMBi had a higher medium protein quantity than the control although not significantly different from the D-Met group. HMBi supplementation stimulated the production of eukaryotic translation initiation factor 3 subunit protein essential for protein translation initiation resulting in higher medium protein synthesis in the HMBi group than in the control group. The protein pathway analysis results showed that the D-Met group stimulated fructose?galactose metabolism, glycolysis pathway, phosphoinositide 3 kinase, and pyruvate metabolism. The HMBi group stimulated the pentose phosphate and glycolysis pathways. Metabolite analysis revealed that the D-Met treatment group increased seven metabolites and decreased uridine monophosphate (UMP) production. HMBi supplementation increased the production of three metabolites and decreased UMP and N-acetyl-L-glutamate production. Taken together, D-Met and HMBi supplementation are effective in stimulating milk protein synthesis in MAC-T cells by genes, proteins, and metabolites stimulation linked to milk protein synthesis.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Enhanced supply of methionine regulates protein synthesis in bovine mammary epithelial cells under hyperthermia condition
한국축산학회지
2021 .09
-
Effect of hyperthermia on cell viability, amino acid transfer, and milk protein synthesis in bovine mammary epithelial cells
한국축산학회지
2022 .01
-
Phenylalanine and valine differentially stimulate milk protein synthetic and energy-mediated pathway in immortalized bovine mammary epithelial cells
한국축산학회지
2020 .01
-
2-DE and MALDI-TOF MS-based identification of bovine whey proteins in milk collected soon after parturition
Korean Journal of Agricultural Science
2018 .12
-
쌀단백질의 가치와 이용방법
식품과학과 산업
2019 .03
-
A TMT-based quantitative proteomic analysis provides insights into the protein changes in the seeds of high- and low- protein content soybean cultivars
Journal of Plant Biotechnology
2020 .01
-
우유의 열처리가 우유품질과 영양가에 미치는 영향: IV. 우유의 열처리가 우유단백질의 이화학적 성질과 영양에 미치는 영향
Journal of Dairy Science and Biotechnology
2017 .01
-
Experimental and Modelling Study of the Denaturation of Milk Protein by Heat Treatment
한국축산식품학회지
2017 .01
-
Profiling of differentially expressed proteins in mature kernels of Korean waxy corn cultivars using proteomic analysis
Applied Biological Chemistry
2015 .01
-
The association between metabolic syndrome and heart failure in middle-aged men and women : population-based study of 2 million individuals
Epidemiology and Health
2022 .01
-
Milk Protein-Stabilized Emulsion Delivery System and Its Application to Foods
Journal of Dairy Science and Biotechnology
2020 .01
-
사료 내 수준별 조단백질 조건에서 보호메티오닌과 보호라이신의 사료첨가 급여가 착유우유생산성 및 유성분에 미치는 영향 : 메타분석
한국유기농업학회지
2016 .01
-
Proteins as the molecular markers of male fertility
식품보건융합연구
2018 .01
-
Monitoring rice anther proteome expression patterns during pollen development
Plant Biotechnology Reports
2020 .01
-
Effects of supplementing limiting amino acids on milk production in dairy cows consuming a corn grain and soybean meal-based diet
한국축산학회지
2020 .01
-
Protein Quality Evaluation of Enzymatic Hydrolyzed Chicken Breast Protein
한국식품영양과학회 학술대회발표집
2021 .10
-
Physicochemical and Functional Properties of Protein Isolates from defatted Sesame and Perilla Seeds
한국식품영양과학회 학술대회발표집
2021 .10
-
Effect of subcritical water process on the structure and antioxidant properties of milk proteins
한국식품영양과학회 학술대회발표집
2022 .10
-
Development and evaluation of probiotic delivery systems using the rennet-induced gelation of milk proteins
한국축산학회지
2021 .09
-
사료 내 단백질과 지방 수준이 참돔(Pagrus major) 치어의 성장, 사료효율 및 비특이적 면역력에 미치는 영향
한국수산과학회지
2015 .06
이 논문의 저자 정보
최근 본 자료
댓글(0)
0