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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.4
- 수록면
- 299 - 306 (8page)
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The transcriptional activator AphB has been implicated in acid resistance and pathogenesis in the food borne pathogens Vibrio vulnificus and Vibrio cholerae. To date, the full-length AphB crystal structure of V. cholerae has been determined and characterized by a tetrameric assembly of AphB consisting of a DNA binding domain and a regulatory domain (RD). Although acidic pH and low oxygen tension might be in-volved in the activation of AphB, it remains unknown which ligand or stimulus activates AphB at the molecular level. In this study, we determine the crystal structure of the AphB RD from V. vulnificus under aerobic conditions without modification at the conserved cysteine residue of the RD, even in the presence of the oxidizing agent cumene hydroperoxide. A cysteine to serine amino acid residue mutant RD protein further confirmed that the cysteine residue is not involved in sensing oxidative stress in vitro. Interestingly, an unidentified small molecule was observed in the inter-subdomain cavity in the RD when the crystal was incubated with cumene hydroperoxide molecules, suggesting a new ligand-binding site. In addition, we confirmed the role of AphB in acid tolerance by observing an aphB-dependent in-crease in cadC transcript level when V. vulnificus was exposed to acidic pH. Our study contributes to the understanding of the AphB molecular mechanism in the process of recognizing the host environment.
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참고문헌 (25)
참고문헌 신청
Adams, P. D. / 2010 / PHENIX: a comprehensive Python-based system for macromolecular structure solution / Acta Crystallogr. D Biol. Crystallogr 66:213~221
Horseman, M. A. / 2011 / A comprehensive review of Vibrio vulnificus: an important cause of severe sepsis and skin and soft-tissue infection / Int. J. Infect Dis 15:e157~e166
Jang, K. K. / 2016 / Regulatory Characteristics of Vibrio vulnificus gbpA Gene Encoding a Mucin-binding Protein Essential for Pathogenesis / J. Biol. Chem 291:5774~5787
Jeong, H. G. / 2008 / Evidence that AphB, essential for the virulence of Vibrio vulnificus, is a global regulator / J. Bacteriol 190:3768~3773
Kantardjieff, K. A. / 2003 / Matthews coefficient probabilities: Improved estimates for unit cell contents of proteins, DNA, and protein-nucleic acid complex crystals / Protein Sci 12:1865~1871
Horseman, M. A. / 2011 / A comprehensive review of Vibrio vulnificus: an important cause of severe sepsis and skin and soft-tissue infection / Int. J. Infect Dis 15:e157~e166
Jang, K. K. / 2016 / Regulatory Characteristics of Vibrio vulnificus gbpA Gene Encoding a Mucin-binding Protein Essential for Pathogenesis / J. Biol. Chem 291:5774~5787
Jeong, H. G. / 2008 / Evidence that AphB, essential for the virulence of Vibrio vulnificus, is a global regulator / J. Bacteriol 190:3768~3773
Kantardjieff, K. A. / 2003 / Matthews coefficient probabilities: Improved estimates for unit cell contents of proteins, DNA, and protein-nucleic acid complex crystals / Protein Sci 12:1865~1871
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