메뉴 건너뛰기
.. 내서재 .. 알림
소속 기관/학교 인증
인증하면 논문, 학술자료 등을  무료로 열람할 수 있어요.
한국대학교, 누리자동차, 시립도서관 등 나의 기관을 확인해보세요
(국내 대학 90% 이상 구독 중)
로그인 회원가입 고객센터 ENG
주제분류

추천
검색

논문 기본 정보

자료유형
학술저널
저자정보
주수지 (한국생명공학연구원) 최승희 (한국생명공학연구원) Jie Eun Yee (Korea Research Institute of Bioscience and Biotechnology) 이옥란 (전남대학교) Kim Suk Weon (Korea Research Institute of Bioscience and Biotechnology)
저널정보
한국식물생명공학회 Plant Biotechnology Reports Plant Biotechnology Reports 제16권 제6호
발행연도
2022.12
수록면
633 - 643 (11page)
DOI
10.1007/s11816-022-00805-6

이용수

표지
📌
연구주제
📖
연구배경
🔬
연구방법
🏆
연구결과
AI에게 요청하기
추천
검색

초록· 키워드

오류제보하기
Phytosulfokines (PSK) belong to a new class of peptide growth factors involved in intercellular signaling in plants. In this study, we examined the efect of PSK on cell division and subsequent adventitious shoot formation in protoplast-derived callus culture of Nicotiana benthamiana. Protoplast cultures and protoplast-derived calluses of N. benthamiana were treated with diferent concentrations of PSK (0, 0.01, 0.03, 0.1, 0.3, and 1 µM). Cell division efciency was the highest at 32% in the 0.1 µM PSK treatment, which was about two times higher than that of control treatment. PSK concentrations<0.1 µM were more efective in inducing cell division in the protoplasts than those>0.3 µM. In contrast, higher concentrations of PSK were more efective in inducing adventitious shoot formation in protoplast-derived calluses. The frequency of adventi tious shoot formation was the highest at 8.5% when the calluses were treated with 1 µM PSK. qRT-PCR analysis showed an increase in the expression of genes involved in cell division and diferentiation: G1/S specifc cyclin (CYCD3-1), cytokinin dependent kinase (CDK), and WUSCHEL (WUS) at 0.1 µM PSK treatment. These results indicate that PSK plays a signifcant role in promoting cell division and adventitious shoot formation in N. benthamiana. The results obtained in this study could be applied to promote cell division and plant regeneration in protoplast cultures of diverse recalcitrant crop plant species.

목차

등록된 정보가 없습니다.

참고문헌 (0)

참고문헌 신청

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!

이 논문의 저자 정보

최근 본 자료

전체보기

댓글(0)

0