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논문 기본 정보

자료유형
학술저널
저자정보
Wang Yuchang (Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of Chin) Shen Bingqi (College of Forestry Northwest A&F University Yangling 712100 Shanxi China) Yang Lin (Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of Chin) Wang Dawei (Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of Chin)
저널정보
한국식물생명공학회 Plant Biotechnology Reports Plant Biotechnology Reports 제16권 제5호
발행연도
2022.10
수록면
553 - 564 (12page)
DOI
10.1007/s11816-022-00771-z

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Yunnanopilia longistaminea is a unique flavor forest vegetable that is mainly determined by the vitamins, amino acids and derivatives, and flavonoids detected in its leaves. This experiment applied to broad targeted metabolomics approach based on UPLC-MS/MS to evaluate metabolic changes and the assessment of differential genes by transcriptome sequencing using the IlluminaHiSeq platform between the Y. longistaminea young leaves (YYL) and mature leaves (YML). The present results showed that a total of 488 compounds in the leaves, of which 275 (56.53%) significantly changed metabolites (SCMs). Compared to young leaves, the abundance of 163 SCMs was down-regulated and 112 SCMs were significantly increased in mature leaves. The results show that flavonoids, amino acids and derivatives, coumarins, nucleotides and derivatives, vitamins, saccharides and alcohol, tannin, and organic acids were significantly downregulated in mature leaves, whereas mature leaves had higher lipids and alkaloids than young leaves. A total of 2082 DEGs were identified, with 939 down- regulated genes and 1143 up-regulated genes in mature leaves compared to young leaves. The experimental work presented here provides the first investigations into Y. longistaminea with metabolic group and transcriptome. This study will prove useful in expanding our understanding of how to analyze biosynthesis at the molecular level, which will encourage further utilization of the Y. longistaminea and its relevant forest vegetables.

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