Evaluation of suitable qRT-PCR normalization genes for various citrus rootstocks

He Wen; Xie Rui; Li Huan; Wang Yan; Chen Qing; Lin Yuanxiu; Zhang Yunting; Luo Ya; Zhang Yong; Tang Haoru; Wang Xiaorong

doi:10.1007/s11816-021-00725-x

추천

검색

자료유형: 학술저널

저자정보: He Wen (College of Horticulture Sichuan Agriculture University) Xie Rui (College of Horticulture Sichuan Agriculture University) Li Huan (College of Horticulture Sichuan Agriculture University) Wang Yan (College of Horticulture Sichuan Agriculture University) Chen Qing (College of Horticulture Sichuan Agriculture University) Lin Yuanxiu (College of Horticulture Sichuan Agriculture University) Zhang Yunting (College of Horticulture Sichuan Agriculture University) Luo Ya (College of Horticulture Sichuan Agriculture University) Zhang Yong (College of Horticulture Sichuan Agriculture University) Tang Haoru (College of Horticulture Sichuan Agriculture University) Wang Xiaorong (College of Horticulture Sichuan Agriculture University)

저널정보: 한국식물생명공학회 Plant Biotechnology Reports Plant Biotechnology Reports 제16권 제1호

발행연도: 2022.2

수록면: 101 - 111 (11page)

DOI: 10.1007/s11816-021-00725-x

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Citrus rootstock can modify plant growth and enhance stress resistance. There are many genotypes and species used as citrus rootstocks. Although multiple citrus rootstocks whole genome sequence and transcriptome databases have previously been published, no suitable internal reference genes have been investigated for standardization of gene expression via quantitative real-time PCR. Here we reported the first systematic and comprehensive analysis of reference genes for quantitative real- time PCR standardization in various citrus rootstocks. The expression stability of ten candidate reference genes in diverse sample subsets of flooding, drought, alkaline and cold treatments was evaluated using four statistical algorithms, geNorm, NormFinder, BestKeeper and ΔCt method. The results demonstrated that the expression stability of reference genes varied under different experimental conditions. In addition, normalization of gene expression of 9-cis-epoxycarotenoid dioxygenase 3 (NCED 3), involved in abscisic acid biosynthesis, was conducted to further confirm the reliability of the reference genes. Overall, EF-1α, DIM 1, GAPC and UBC expressed much more stably. ACTIN and GAPDH were not recommended for normalization in given experimental conditions due to low stability. Our main contribution was to identify reference genes with suitable and stable expression in citrus rootstocks varied across selected experimental conditions. Furthermore, these results will provide valuable information for future research on citrus rootstocks.

#Citrus rootstock?· Reference gene?· Abiotic stress?· Gene expression?· Normalization

참고문헌 (0)

참고문헌 신청

참고문헌이 DBpia에서 서비스 중이라면, [참고문헌 신청]을 통해 등록해보세요

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!