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논문 기본 정보

자료유형
학술저널
저자정보
Kyoung Sook Cho (Department of Bioengineering Pukyong National University) 김중균 (부경대학교)
저널정보
한국수산과학회 Fisheries and Aquatic Sciences Fisheries and Aquatic Sciences 제25권 제10호
발행연도
2022.10
수록면
525 - 536 (12page)

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Photosynthetic bacteria (PSB) attract considerable interest as useful microorganisms; nevertheless, a generalized culture technique has not been previously reported owing to difficulty in their cultivation. Therefore, a simple culture technique suitable for public use was investigated. Among the PSB tested, the strain Rhodobacter azotoformans EBN-7 was the most suitable for scaleup production because it showed the highest specific growth rate (0.20 h–1) on basal medium. In scale-up cultivation (500 L), R. azotoformans EBN-7 showed 4.50 × 1010 colony-forming units mL–1 (number of viable cells), dry cell weight of 26.8 g/L, and a specific growth rate of 0.15 h–1. Cultivation using this final culture broth (as seed culture) in a 15 L simple reactor was successful, with maintenance of cell activity evident. For use as seed culture, the maximum allowable preservation period of R. azotoformans EBN-7 at 4℃ was 3 months. When R. azotoformans EBN-7 cultivated in a simple technique was applied to shrimp aquaculture water, NH4 +-N was reduced from 0.61 mg/L to 0.24 mg/L (by 60.7%) in 4 days in comparison with the control. Thus, this simple culture technique using R. azotoformans EBN-7 has the potential for a good removal efficiency of NH4 +-N, making seed culture easier and suitable for public use.

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