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논문 기본 정보

자료유형
학술저널
저자정보
Suriyachadkun Chanwit (Thailand Bioresource Research Center National Center for Genetic Engineering and Biotechnology (BIO) Chunhachart Orawan (Division of Microbiology Department of Science Faculty of Liberal Arts and Science Kasetsart Univer) Srithaworn Moltira (Division of Microbiology Department of Science Faculty of Liberal Arts and Science Kasetsart Univer) Tangchitcharoenkhul Rungnapa (Graduate School Suan Dusit University Bangkok 10300 Thailand) Tangjitjareonkun Janpen (Department of Resources and Environment Faculty of Science at Sriracha Kasetsart University Srirach)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제32권 제11호
발행연도
2022.11
수록면
1,435 - 1,446 (12page)
DOI
10.4014/jmb.2206.06058

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Zinc-solubilizing bacteria can convert the insoluble form of zinc into soluble forms available to plants. This study was conducted to isolate and screen zinc-solubilizing actinobacteria from rhizosphere soils and to assess their effect on vegetable soybean growth. In total, 200 actinobacteria strains belonging to 10 genera were isolated from rhizosphere soil samples. Among these isolates, four showed zinc solubilization with solubilizing index values ranging from 3.11 to 3.78 on Bunt and Rovira agar supplemented with 0.1% zinc oxide. For the quantitative assay, in broth culture, strains CME34 and EX51 solubilized maximum available zinc contents of 529.71 and 243.58 μg/ml. Furthermore, indole-3-acetic acid (IAA) and ammonia were produced by these two strains, the strain CME34 produced the highest amount of IAA 4.62 μg/ml and the strain EX51 produced the highest amount of ammonia 361.04 μg/ml. In addition, the phosphate-solubilizing abilities in Pikovskaya’s medium of CME34 and EX51 were 64.67 and 115.67 μg/ml. Based on morphological and biochemical characterization and 16S rDNA sequencing, the strains CME34 and EX51 were closely related to the genus Streptomyces. In a greenhouse experiment, single-strain inoculation of Streptomyces sp. CME34 or EX51 significantly increased the shoot length, root length, plant dry weight, number of pods per plant and number of seeds per plant of vegetable soybean plants compared to the uninoculated control. These findings facilitated the conclusion that the two Streptomyces strains have potential as zinc solubilizers and can be suggested as bioinoculants to promote the growth and yield of soybean.

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