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논문 기본 정보

자료유형
학술저널
저자정보
Tang Qiwu (Hunan Biological and Electromechanical Polytechnic Changsha 410128 P.R. China) Deng Zaofu (Hunan Biological and Electromechanical Polytechnic Changsha 410128 P.R. China) Tan Shengguo (Hunan Biological and Electromechanical Polytechnic Changsha 410128 P.R. China) Song Guo (Animal Husbandry and Fishery Bureau of Ningyuan Yongzhou 425000 P.R. China) Zhang Hai (Animal Epidemic Prevention Station of Xiangxi Autonomous Prefecture Xiangxi 416000 P.R. China) Ge Lingrui (Hunan Biological and Electromechanical Polytechnic Changsha 410128 P.R. China)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제32권 제9호
발행연도
2022.9
수록면
1,120 - 1,125 (6page)
DOI
10.4014/jmb.2207.07068

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Japanese encephalitis virus (JEV), the causative agent of Japanese encephalitis (JE), is an importantly zoonotic, vector-borne virus widely prevalent in Asia. Although JE has been well controlled in China, its prevalence remains a huge threat to the pig industry as well as human health. Herein, we report on our molecular and serological investigations of JEV among pigs from different regions in Hunan Province of China from 2019 to 2021. Collectively, 19.27% (583/3026, 95% Confidential Interval (CI) 17.86-20.68) of sampled pigs were positive for JEV IgG antibody as revealed by indirect enzyme-linked immunosorbent assay, and the seroprevalence of JEV among pigs was significantly associated with the development stage and breeding scale (p < 0.01). Meanwhile, 10.99% (42/382, 95% CI 7.86-14.13) of tissue samples of pigs with suspected clinical symptoms of JE and 23.44% (15/64, 95% CI 13.06-33.82) of mosquito batches were JEV-positive via reverse polymerase chain reaction. In addition, the complete E gene sequences of 14 JEV strains identified in this study were amplified and sequenced. Phylogenetic analysis showed that all 14 JEV strains belonged to genotype I-b and displayed a distinct genetic relationship to the present JEV vaccine strain (SA14-14-2). In conclusion, our results revealed not only the severe prevalence of JEV in Hunan Province, but also that JEV I-b might be the predominant genotype in Hunan Province, suggesting therefore that effective measures for JE control are urgently needed.

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