지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2022.11
- 수록면
- 136 - 145 (10page)
이용수
이 논문의 연구 히스토리 (2)
- 이 논문의 후속연구가 궁금하신가요?
- 연관 학술논문 또는 학술발표를 통해 보다 발전된 연구결과를 확인하실 수 있습니다.
- 이 논문의 연구 히스토리 확인하기
초록· 키워드
오류제보하기
Later flow immunoassay (LFIA) is a protein analytical method based on immunoreaction. On the LFIA based protein analytical method, bioreceptor molecule plays a key role, and so a system that evaluates and manages the binding affinity of bioreceptor is needed to secure detection reliability. In this study, Lateral Flow Immunoassay based rapid Bioreceptor Screening Method (rBSM) is presented that provide a simple and quick evaluating method for the binding affinity to the target protein of the antibody as model bioreceptor. To verify this evaluation method, Virus-like particles (VLP) and anti-VLP antibodies are selected as a model norovirus, which is target protein, and the candidate bioreceptors respectively. Among the 5 different candidate antibodies, appropriate antibody could be sorted out within 30 minutes through rBSM. In addition, selected antibodies were applied to two representative LFIA based techniques, sandwich assay and competitive assay. Among these methods, sandwich assay showed more effective VLP detection method. Through applying selected antibodies and techniques to the commercialized mass production lines, an VLP detecting LFIA kit was developed with a detection limit of 1012 copies/g of VLPs in real samples. Since this proposed method in this study could be easily transformable into other combinations with bioreceptors, it is expected that this technique would be applied to LFIA kit development system and bioreceptor quality management.
목차
Abstract
1. 서론
2. 실험장치 및 방법
3. 결과 및 고찰
4. 결론
REFERENCE
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
노로바이러스 검출을 위한 측면유동면역분석법 기반의 바이오리셉터 선별기법 개발
한국가시화정보학회 학술발표대회 논문집
2022 .12
-
The Rapid Bioreceptor Screening Method based on Lateral Flow Immunoassay
한국생물공학회 학술대회
2023 .10
-
측방 유동면역분석(LFIA)의 인플루엔자-A 항원농도 예측 모델 개발
한국통신학회 인공지능 학술대회 논문집
2023 .09
-
측면유동면역분석 (LFIA) 스트립에서의 고흡수성수지(SAP)를 활용한 생체분자에 대한 가시화된 검출 신호 향상
한국가시화정보학회 학술발표대회 논문집
2022 .05
-
Enhancing the Sensitivity of Lateral Flow Immunoassay by Engineered Cellulose-binding Protein Linker Fused with Antibody-binding Domains
한국생물공학회 학술대회
2021 .10
-
Lateral Flow Immunoassay Based on Gold Nanoparticles for Rapid and Sensitive On-site Detection of Histamine
한국생물공학회 학술대회
2020 .06
-
Rapid Detection of Ractopamine Residues through Antibody-conjugated Gold Nanoparticles based Lateral Flow Immunoassay Strip
한국생물공학회 학술대회
2020 .06
-
Development of later flow assay (LFA) kit for the detection of harmful factors in food
한국생물공학회 학술대회
2018 .10
-
Rapid and sensitive detection of the histamine on lateral flow immunoassay based strip sensor in food
한국생물공학회 학술대회
2018 .10
-
Oriented Antibody Immobilization-based Lateral Flow Immunoassay for Sensitive Detection of SARS-CoV-2
한국생물공학회 학술대회
2023 .04
-
Development of Lateral Flow Assays with Magnetic Nanoparticles for Quantitative Antibody Detection
대한기계학회 춘추학술대회
2022 .05
-
구를 사용한 VLP-16 라이다 센서와 다시점 카메라 간의 보정
대한전자공학회 학술대회
2017 .06
-
On-site Rapid Detection of Ractopamine Residues in Pork Using Lateral Flow Immunoassay Strips
한국생물공학회 학술대회
2020 .10
-
Engineered carbohydrate-binding protein with antibody-immobilizing ability for improving performance of paper-based immunoassays
한국생물공학회 학술대회
2019 .04
-
Gold Nanoparticle Based Lateral Flow Immunoassay for the Rapid and Sensitive Detection of Ractopamine in Pork
한국생물공학회 학술대회
2021 .04
-
Development of a SARS-CoV-2-specific Biosensor for Antigen Detection Using scFv-Fc Fusion Proteins
한국생물공학회 학술대회
2021 .04
-
Development and Commercialization of Magnetic Force-Assisted Electrochemical Sandwich Immunoassay (MESIA) for Point-of-Care Testing
한국생물공학회 학술대회
2021 .04
-
Developing a Recombinant Secondary Antibody Mimic as Signal Amplifier in Immunoassays
한국생물공학회 학술대회
2019 .04
-
Gold Nanoparticle Based Lateral Flow Immunoassay for the Rapid and Sensitive Detection of Ractopamine in Pork
한국생물공학회 학술대회
2021 .10
-
Anti-SARS-CoV-2 Nucleoprotein Antibodies Derived from Pig Serum with a Controlled Specificity
BioChip Journal
2021 .01
이 논문의 저자 정보
이 논문과 함께 이용한 논문
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2023-505-000233943