지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.2
- 수록면
- 27 - 32 (6page)
이용수
초록· 키워드
오류제보하기
We present an autofocus tracking system implemented by the digital refocusing of digital holographic microscopy (DHM) and the tunability of an electrically tunable lens (ETL). Once the defocusing distance of an image is calculated with the DHM, then the focal plane of the imaging system is optically tuned so that it always gives a well-focused image regardless of the object location. The accuracy of the focus is evaluated by calculating the contrast of refocused images. The DHM is performed in an off-axis holographic configuration, and the ETL performs the focal plane tuning. With this proposed system, we can easily track down the object drifting along the depth direction without using any physical scanning. In addition, the proposed system can simultaneously obtain the digital hologram and the optical image by using the RGB channels of a color camera. In our experiment, the digital hologram is obtained by using the red channel and the optical image is obtained by the blue channel of the same camera at the same time. This technique is expected to find a good application in the long-term imaging of various floating cells.
목차
I. INTRODUCTION
II. METHODS
III. RESULTS
IV. CONCLUSION
REFERENCES
참고문헌 (29)
참고문헌 신청
J. -M. Geusebroek / 2000 / Robust autofocusing in microscopy / Cytometry 39:1~9
G. Rabut / 2004 / Automatic real-time threedimensional cell tracking by fluorescence microscopy / J. Micros 216:131~137
R. Redondo / 2012 / Autofocus evaluation for brightfield microscopy pathology / J. Biomed. Opt 17:036008
M. E. Bravo-Zanoguera / 2007 / Dynamic autofocus for continuousscanning time-delay-and-integration image acquisition in automated microscopy / J. Biomed. Opt 12:034011
M. Duocastella / 2012 / Simultaneous imaging of multiple focal planes for three-dimensional microscopy using ultra-high-speed adaptive optics / J. Biomed. Opt 17:050505
G. Rabut / 2004 / Automatic real-time threedimensional cell tracking by fluorescence microscopy / J. Micros 216:131~137
R. Redondo / 2012 / Autofocus evaluation for brightfield microscopy pathology / J. Biomed. Opt 17:036008
M. E. Bravo-Zanoguera / 2007 / Dynamic autofocus for continuousscanning time-delay-and-integration image acquisition in automated microscopy / J. Biomed. Opt 12:034011
M. Duocastella / 2012 / Simultaneous imaging of multiple focal planes for three-dimensional microscopy using ultra-high-speed adaptive optics / J. Biomed. Opt 17:050505
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UCI(KEPA) : I410-ECN-0101-2023-425-000232249