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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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This paper describes a comparative analysis of yeast cell viability at exponential and stationary growth phases using multiple conventional techniques and statistical tools. Overall, cellular responses to various viability assays were asynchronous. Results of optical density measurement and direct cell counting were asynchronous both at exponential and stationary phases. Proliferative capacity measurement using SP-SDS indicated that cells at the end of the stationary phase were proliferative as much as exponentially growing cells. Metabolic activity assays using two different dyes concluded that the inside of cells at stationary phase is slightly less reducing compared to that of exponentially growing cells, implying that the metabolic activity imperceptibly declined as cells were aged. These results will be helpful to understand the details of yeast cell viability at exponential and stationary growth phases.
목차
Abstract
1. Introduction
2. Experimental
3. Results and Discussion
4. Conclusions
References
참고문헌 (23)
참고문헌 신청
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G. Cazzanelli, F. Pereira, S. Alves, R. Francisco, L. Azevedo, P. Dias Carvalho, A. Almeida, M. Côrte-Real, M. J. Oliveira, C. Lucas, M. J. Sousa and A. Preto, Cells, 7(2), 14 (2018).
N. Nagaraj, N. Alexander Kulak, J. Cox, N. Neuhauser, K. Mayr, O. Hoerning, O. Vorm and M. Mann, Molecular & Cellular Proteomics, 11(3), M111.013722 (2012).
S. Ostergaard, L. Olsson and J. Nielsen, Microbiology and Molecular Biology Reviews, 64(1), 34-50 (2000).
J. Förster, I. Famili, P. Fu, B. Ø. Palsson and J. Nielsen, Genome Research, 13(2), 244-253 (2003).
G. Cazzanelli, F. Pereira, S. Alves, R. Francisco, L. Azevedo, P. Dias Carvalho, A. Almeida, M. Côrte-Real, M. J. Oliveira, C. Lucas, M. J. Sousa and A. Preto, Cells, 7(2), 14 (2018).
N. Nagaraj, N. Alexander Kulak, J. Cox, N. Neuhauser, K. Mayr, O. Hoerning, O. Vorm and M. Mann, Molecular & Cellular Proteomics, 11(3), M111.013722 (2012).
S. Ostergaard, L. Olsson and J. Nielsen, Microbiology and Molecular Biology Reviews, 64(1), 34-50 (2000).
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