지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Objectives: Bee venom (BV) is a widely used therapy in Traditional East Asian Medicine (TEAM). We previously reported that BV was clinically effective for treating Parkinson’s disease, that phospholipase A₂ (PLA₂) was the main component of BV, and that it induced regulatory T cells (Tregs) by binding CD206 on dendritic cells (DCs). Therefore, we aimed to reconfirm our findings in human blood samples and investigate the relationship between CD206<SUP>+</SUP> DCs and clinical syndrome differentiation in TEAM.
Methods: We surveyed 100 subjects with questionnaires on cold-heat patternization and obtained their blood samples. The obtained human peripheral blood monocytes (hPBMCs) were washed with phosphate-buffered saline (PBS). After resuspension with ex vivo media, numbers of cells were counted. Tregs were counted after culturing the samples in a 37℃ CO₂ incubator for 72 h.
Results: We divided the subjects into a relatively high CD206<SUP>+</SUP> group or a relatively low CD206<SUP>+</SUP> group. The heat factor scores of high CD206<SUP>+</SUP> group were significantly higher than that of low CD206<SUP>+</SUP> group (high vs low: 239.2 ± 54.1 vs 208.4 ± 55.1, p=0.023). After culturing with PLA₂, Tregs increased in the high CD206<SUP>+</SUP> group but decreased in the low CD206+ group.
Conclusion: In this study, we reconfirm that CD206<SUP>+</SUP> DCs induced Treg differentiation by incubating human blood samples with PLA₂ and that they showed an association with syndrome differentiation, especially with heat patterns, in TEAM. A heat pattern in TEAM might be one indication for PLA₂ therapy because its score was elevated in the high CD206<SUP>+</SUP> group.
Methods: We surveyed 100 subjects with questionnaires on cold-heat patternization and obtained their blood samples. The obtained human peripheral blood monocytes (hPBMCs) were washed with phosphate-buffered saline (PBS). After resuspension with ex vivo media, numbers of cells were counted. Tregs were counted after culturing the samples in a 37℃ CO₂ incubator for 72 h.
Results: We divided the subjects into a relatively high CD206<SUP>+</SUP> group or a relatively low CD206<SUP>+</SUP> group. The heat factor scores of high CD206<SUP>+</SUP> group were significantly higher than that of low CD206<SUP>+</SUP> group (high vs low: 239.2 ± 54.1 vs 208.4 ± 55.1, p=0.023). After culturing with PLA₂, Tregs increased in the high CD206<SUP>+</SUP> group but decreased in the low CD206+ group.
Conclusion: In this study, we reconfirm that CD206<SUP>+</SUP> DCs induced Treg differentiation by incubating human blood samples with PLA₂ and that they showed an association with syndrome differentiation, especially with heat patterns, in TEAM. A heat pattern in TEAM might be one indication for PLA₂ therapy because its score was elevated in the high CD206<SUP>+</SUP> group.
목차
Introduction
Methods
Results
Discussion
References
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