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논문 기본 정보

자료유형
학술저널
저자정보
저널정보
한국동물위생학회(구 한국가축위생학회) 한국가축위생학회지 한국가축위생학회지 제38권 제1호
발행연도
2015.1
수록면
25 - 30 (6page)

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초록· 키워드

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Bee venom has been used as to prevent and treat bovine mastitis as natural antimicrobial compoundsin some dairy cattle farms in Korea. It is needed to determine the residual of bee venom in milks ofdairy cattle treated with bee venom. Since bee venom is not approved as a raw material for animaldrugs, the preprocessing method to detect bee venom residual in milk and the tolerance limit for itsresidue has not been established yet in Korea. Therefore, the purpose of this study was to developpre-processing method not affecting major component of bee venom for detection of its residue in milksusing ultra-high performance liauid chromatography (UHPLC). In addition, bee venom residue was alsoanalyzed in milk samples of dairy cattle treated for mastitis with bee venom using UHPLC with thedeveloped pre-processing method in this study. As a result, melittin, histamin and phospolipase A2, themajor components of bee venom, were all detected by UHPLC with the pre-processing method developedin this study. The results of this study suggest that the pre-processing method developed in thisstudy can be useful to detect bee venom residue in dairy cattle milk. We also found that no bee venomresidues were detected in milk samples collected from dairy cattle treated with bee venom after 1 and3 days, respectively.

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