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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한의용생체공학회 Biomedical Engineering Letters (BMEL) Biomedical Engineering Letters (BMEL) Vol.5 No.1
- 발행연도
- 2015.1
- 수록면
- 58 - 64 (7page)
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초록· 키워드
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Purpose To improve effect of liver disease treatment, tissueengineering approach such as direct hepatocyte injection hasbeen investigated. Encapsulation, mixing cells and biomaterialsto enclose cells within a biomaterial capsule, is commonlyused to deliver cells into the body. Many kinds of biomaterialsincluding natural and artificial materials have been used. Thecapsule must have biocompatibility and microstructure forcell culture, survival and proliferation as well as cell functionand therapeutic effects. However, most biomaterials usedfor encapsulation have low biocompatibility, insufficientconstituents and an unsuitable 3-dimensional structure. Tosolve these problems, we performed encapsulation using adecellularized liver scaffold (DCLS) with a native extracellularmatrix (ECM) and natural porous microstructure includingvasculature.
Methods DCLS was prepared with 0.1% sodium dodecylsulfate under agitation and 2 mm2 sized DCLS pieces weresterilized with peracetic acid (25.6 μl/10 ml) for 24 hours.
Histological analysis showed that the DCLS had nativeECM, liver specific major architecture and blood vesselstructure but no cells. For cell encapsulation, hepG2 cellswere injected into DCLS pieces with a syringe and culturedfor 5 days.
Results The cells survived and formed a cell mass with aliver ECM microstructure inside the DCLS capsules. Theencapsulation status was similar to capsules formed by currentencapsulation techniques.
Conclusions DCLS can be used to make an encapsulationcell delivery system.
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참고문헌 (43)
참고문헌 신청
Elizabeth J / 2008 / Cellular transplantation for liver diseases / Gastroenterol Res 1(1):8~13
Dixit V / 1990 / Restoration of liver function in gunn rats without immunosuppression using transplanted microencapsulated hepatocytes / Hepatology 12(6):1342~1349
Zhao Y / 2010 / In vivo generation of thick, vascularized hepatic tissue from collagen hydrogel-based hepatic units / Tissue Eng Part C Methods 16(4):653~659
Fan J / 2010 / Preparation and characterization of chitosan/galactosylated hyaluronic acid scaffolds for primary hepatocytes culture / J Mater Sci Mater Med 21(1):319~327
Semino CE / 2003 / Functional differentiation of hepatocyte-like spheroid structures from putative liver progenitor cells in three-dimensional peptide scaffolds / Differentiation 71(4-5):262~270
Dixit V / 1990 / Restoration of liver function in gunn rats without immunosuppression using transplanted microencapsulated hepatocytes / Hepatology 12(6):1342~1349
Zhao Y / 2010 / In vivo generation of thick, vascularized hepatic tissue from collagen hydrogel-based hepatic units / Tissue Eng Part C Methods 16(4):653~659
Fan J / 2010 / Preparation and characterization of chitosan/galactosylated hyaluronic acid scaffolds for primary hepatocytes culture / J Mater Sci Mater Med 21(1):319~327
Semino CE / 2003 / Functional differentiation of hepatocyte-like spheroid structures from putative liver progenitor cells in three-dimensional peptide scaffolds / Differentiation 71(4-5):262~270
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