지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한백신학회 Clinical and Experimental Vaccine Research Clinical and Experimental Vaccine Research Vol.7 No.2
- 발행연도
- 2018.1
- 수록면
- 129 - 138 (10page)
이용수
초록· 키워드
오류제보하기
Purpose: Study on the pathogen and the pathogen-related disease require the information at both cellular and organism level. However, lack of appropriate high-quality antibodies and the difference between the experimental animal models make it difficult to analyze in vivo mechanism of pathogen-related diseases. For more reliable research on the infection and immune-response of pathogen-related diseases, accurate analysis is essential to provide spatiotemporal information of pathogens and immune activity to avoid false-positive or mis-interpretations. In this regards, we have developed a method for tracking Francisella tularensis in the animal model without using the specific antibodies for the F. tularensis. Materials and Methods: A dual reporter plasmid using GFP-Lux with putative bacterioferritin promoter (pBfr) was constructed and transformed to F. tularensis live vaccine strain to generate F. tularensis LVS (FtLVS)-GFP-Lux for both fluorescence and bioluminescence imaging. For vaccination to F. tularensis infection, FtLVS and lipopolysaccharide (LPS) from FtLVS were used. Results: We visualized the bacterial replication of F. tularensis in the cells using fluorescence and bioluminescence imaging, and traced the spatio-temporal process of F. tularensis pathogenesis in mice. Vaccination with LPS purified from FtLVS greatly reduced the bacterial replication of FtLVS in animal model, and the effect of vaccination was also successfully monitored with in vivo imaging Conclusion: We successfully established dual reporter labeled F. tularensis for cellular and whole body imaging. Our simple and integrated imaging analysis system would provide useful information for in vivo analysis of F. tularensis infection as well as in vitro experiments, which have not been fully explained yet with various technical problems.
목차
등록된 정보가 없습니다.
참고문헌 (16)
참고문헌 신청
Meighen EA / 1991 / Molecular biology of bacterial bioluminescence / Microbiol Rev 55:123~142
Golovliov I / 2003 / An attenuated strain of the facultative intracellular bacterium Francisella tularensis can escape the phagosome of monocytic cells / Infect Immun 71:5940~5950
Bina XR / 2010 / Construction of a bioluminescence reporter plasmid for Francisella tularensis / Plasmid 64:156~161
Giorno R / 2009 / Localization and assembly of proteins comprising the outer structures of the Bacillus anthracis spore / Microbiology 155:1133~1145
Glomski IJ / 2007 / Primary involvement of pharynx and peyer's patch in inhalational and intestinal anthrax / PLoS Pathog 3:e76
Golovliov I / 2003 / An attenuated strain of the facultative intracellular bacterium Francisella tularensis can escape the phagosome of monocytic cells / Infect Immun 71:5940~5950
Bina XR / 2010 / Construction of a bioluminescence reporter plasmid for Francisella tularensis / Plasmid 64:156~161
Giorno R / 2009 / Localization and assembly of proteins comprising the outer structures of the Bacillus anthracis spore / Microbiology 155:1133~1145
Glomski IJ / 2007 / Primary involvement of pharynx and peyer's patch in inhalational and intestinal anthrax / PLoS Pathog 3:e76
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Immune response to synthetic subunit peptides, Tul4 and FopA, of Francisella tularensis
한국실험동물학회 학술발표대회 논문집
2015 .02
-
Immune response to synthetic subunit peptides, Tul4 and FopA, of Francisella tularensis
한국실험동물학회 학술발표대회 논문집
2015 .08
-
Humanized Animal Models for Studying the Efficacy of Vaccines Against Tularemia
한국실험동물학회 학술발표대회 논문집
2016 .02
-
The first pediatric case of tularemia in Korea: manifested with pneumonia and possible infective endocarditis
Clinical and Experimental Pediatrics
2015 .01
-
Visualizing biological phenomena by fluorescent molecular spies
한국실험동물학회 학술발표대회 논문집
2015 .02
-
Application of optical small animal imaging in preclinical research
한국실험동물학회 학술발표대회 논문집
2021 .07
-
Ex vivo culture conditions for bioluminescence imaging of porcine skin tissue
Journal of Biomedical and Translational Research
2024 .03
-
[우수신진연구자 발표 2] Establishment of cell-type-specific inflammation in vivo imaging mouse model based on NF-kB-driven luciferase
한국실험동물학회 학술발표대회 논문집
2022 .01
-
[해부생리] Dual utility NF-κB reporter mouse for non-invasive monitoring of ubiquitous and cell-type-specific inflammation
한국실험동물학회 학술발표대회 논문집
2022 .01
-
Microbiological survey of Korean animal facilities from 2014 to 2019
한국실험동물학회 학술발표대회 논문집
2021 .07
-
Bioluminescence and fluorescence imaging of lipopolysaccharide-induced endotoxemic mice
한국실험동물학회 학술발표대회 논문집
2017 .02
-
Molecular imaging of atherosclerosis using reporter gene system
대한방사성의약품학회지
2018 .01
-
Intravital confocal & two-photon microscopy for in vivo cellular-level imaging of live animal model
한국실험동물학회 학술발표대회 논문집
2025 .02
-
Establishment of animal models using molecular imaging
한국실험동물학회 학술발표대회 논문집
2015 .02
-
Future Perspectives on New Approaches in Pathogen Detection
대한의생명과학회지
2015 .12
-
Analysis of Antibody Responses After COVID-19 Vaccination in Liver Transplant Recipients: A Single-Center Study
Journal of Korean Medical Science
2023 .04
-
In vivo visualization of cellular dynamics in living animal
한국실험동물학회 학술발표대회 논문집
2025 .02
-
Molecular Imaging in the Era of Personalized Medicine
Journal of Pathology and Translational Medicine
2015 .01
-
Biological applications of micro-architecture by X-ray computed micro- and nano-tomography
한국실험동물학회 학술발표대회 논문집
2021 .07
-
In vivo imaging-aided cancer gene therapy
한국실험동물학회 학술발표대회 논문집
2016 .08
이 논문의 저자 정보
최근 본 자료
댓글(0)
0