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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 567 - 577 (11page)
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We aim to examine the influence of platelet rich plasma (PRP) and spatial cues in cartilage/bone matrix forming proteins, and to evaluate the mitotic and chemotactic potential of PRP on human mesenchymal stem cells (hMSCs). Directed cell migration towards PRP gradients was assessed in chemotactic chambers, and recorded by time-lapse microscopy. hMSCs cultured in three-dimensional (3D) scaffolds were visualized by scanning electron microscopy; Hoechst dye was used to confirm cell confluence in 3D-constructs and monolayers before experimental treatment. MSCs were treated with 10% PRP lysate or 10% PRP lysate supplemented with TGF-b-based differentiation medium. The expression of cartilage (COL2A1, Sox9, ACAN, COMP), and bone (COL1A1, VEGF, COL10A1, Runx2) fundamental genes was assessed by real time PCR in monolayers and 3D-constructs. PRP had mitotic (p\0.001), and chemotactic effect on hMSCs, Ralyleigh test p = 1.02E - 10. Two and three-week exposure of MSCs to PRP secretome in 3Dconstructs or monolayers decreased Sox9 expression (p\0.001 and p = 0.050) and COL2A1, (p = 0.011 and p = 0.019). MSCs in monolayers exposed to PRP showed increased ACAN (p = 0.050) and COMP (p\0.001). Adding TGF-b-based differentiation medium to PRP increased COMP, and COL2A1 expression at gene and protein level, but merely in 3D-constructs, p\0.001. TGF-b addition to monolayers reduced Sox9 (p\0.001), aggrecan (p = 0.004), and VEGF (p = 0.004). Cells exposed to PRP showed no changes in hypertrophy associated genes in either monolayers or 3Dconstructs. Our study suggests hMSCs have high-degree of plasticity having the potential to change their matrix-forming phenotype when exposed to PRP and according to spatial configuration.
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참고문헌 (35)
참고문헌 신청
Murray CJ / 2013 / Measuring the global burden of disease / N Engl J Med 369:448~457
Losina E / 2013 / Lifetime risk and age at diagnosis of symptomatic knee osteoarthritis in the US / Arthritis Care Res(Hoboken) 65:703~711
Andia I / 2015 / Meeting current musculoskeletal health demand through deeper insights into tissue homeostasis and regeneration / Expert Opin Biol Ther 15:767~771
Brittberg M / 1994 / Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation / N Engl J Med 331:889~895
Nejadnik H / 2010 / Autologous bone marrow-derived mesenchymal stem cells versus autologous chondrocyte implantation : an observational cohort study / Am J Sports Med 38:1110~1116
Losina E / 2013 / Lifetime risk and age at diagnosis of symptomatic knee osteoarthritis in the US / Arthritis Care Res(Hoboken) 65:703~711
Andia I / 2015 / Meeting current musculoskeletal health demand through deeper insights into tissue homeostasis and regeneration / Expert Opin Biol Ther 15:767~771
Brittberg M / 1994 / Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation / N Engl J Med 331:889~895
Nejadnik H / 2010 / Autologous bone marrow-derived mesenchymal stem cells versus autologous chondrocyte implantation : an observational cohort study / Am J Sports Med 38:1110~1116
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