지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 253 - 265 (13page)
이용수
초록· 키워드
오류제보하기
In recent years, several kinds of cardiac progenitor cells have been identified and isolated from heart tissue. These cells showed differentiation potential into cardiomyocytes, smooth muscle cells, and endothelial cells in vitro and in vivo. Morphogenetic events are tightly regulated during development to determine cell destiny and reshape the embryonic lineage. In this study, we directly compared the characteristics of rat fetal cardiac progenitor cells (rFCPCs) isolated from the chamber formation stage at embryonic day 12 (E12) and at the septation stage of E15. Both kinds of rFCPCs expressed mesenchymal stem cell markers (CD105, CD73, and CD29) but not CD34 and CD45. The E12 rFCPCs expressed a high level of Oct4 compared to E15 until passage 5 and showed a steep decline of Nkx2.5 expression at passage 5. However, Nkx2.5 expression at E15 was maintained until passage 5 and Oct4 expression slightly increased at passage 5. We also detected an intense staining for Oct4 antibody in E12 heart tissue sections. The average doubling time of the E12 rFCPCs from passage 3 to passage 15 was about 5 hours longer than E15. These cells could also be induced into cardiomyocytes expressing a-MHC, cTnT, cTnC, and Cx43 under cardiomyogenic culture conditions and rFCPCs at E15 showed more intense staining of a-MHC than cells at E12 by immunocytochemistry. Taken together, our results show that developmental differences between E12 and E15 may influence their properties and differentiation. Furthermore those differences should be considered when deciding on the optimal cell source for cell replacement therapy in cardiovascular regeneration.
목차
등록된 정보가 없습니다.
참고문헌 (49)
참고문헌 신청
Matar AA / 2014 / Stem cell therapy for cardiac dysfunction / Springerplus 3:440
Kuhn EN / 2010 / Origin of cardiac progenitor cells in the developing and postnatal heart / J Cell Physiol 225:321~325
Lunde K / 2006 / Intracoronary injection of mononuclear bone marrow cells in acute myocardial infarction / N Engl J Med 355:1199~1209
Scha¨chinger V / 2006 / Improved clinical outcome after intracoronary administration of bone-marrowderived progenitor cells in acute myocardial infarction : final 1-year results of the REPAIR-At55MI trial / Eur Heart J 27:2775~2783
Rose RA / 2008 / Bone marrow-derived mesenchymal stromal cells express cardiac-specific markers, retain the stromal phenotype, and do not become functional cardiomyocytes in vitro / Stem Cells 26:2884~2892
Kuhn EN / 2010 / Origin of cardiac progenitor cells in the developing and postnatal heart / J Cell Physiol 225:321~325
Lunde K / 2006 / Intracoronary injection of mononuclear bone marrow cells in acute myocardial infarction / N Engl J Med 355:1199~1209
Scha¨chinger V / 2006 / Improved clinical outcome after intracoronary administration of bone-marrowderived progenitor cells in acute myocardial infarction : final 1-year results of the REPAIR-At55MI trial / Eur Heart J 27:2775~2783
Rose RA / 2008 / Bone marrow-derived mesenchymal stromal cells express cardiac-specific markers, retain the stromal phenotype, and do not become functional cardiomyocytes in vitro / Stem Cells 26:2884~2892
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
심근유래의 줄기세포 프라이밍 및 조직공학 기술에 의한 심혈관 재생 전략
KSBB Journal
2018 .09
-
Isolation and Identification of Mesenchymal Stem Cells from Human Mastoid Bone Marrow
조직공학과 재생의학
2015 .01
-
Transcriptional regulatory network for the establishment of CD8+ T cell exhaustion
Experimental and Molecular Medicine
2021 .02
-
Altered lipid metabolism promoting cardiac fibrosis is mediated by CD34+ cell-derived FABP4+ fibroblasts
Experimental and Molecular Medicine
2024 .08
-
Development of culture strategies for stem cell maintenance and differentiation through regulation of cell behaviors
한국생물공학회 학술대회
2016 .04
-
Analyses of the TCR repertoire of MHC class II-restricted innate CD4+ T cells
Experimental and Molecular Medicine
2015 .01
-
Human Cell Atlas and cell-type authentication for regenerative medicine
Experimental and Molecular Medicine
2020 .09
-
Three-Dimensional Cell Culture Using Modified Natural Polymer for Cell Therapy
한국생물공학회 학술대회
2020 .06
-
The expression and functional roles of microRNAs in stem cell differentiation
BMB Reports
2016 .01
-
Chronic LCMV infection regulates the effector T cell response by inducing the generation of less immunogenic dendritic cells
Experimental and Molecular Medicine
2023 .05
-
Development of Next-generation Cell-based Therapeutics for Diabetes
한국생물공학회 학술대회
2023 .04
-
FGF2-Mediated Insulin-Producing Beta Cells Differentiated from Human Omentum-Derived Stem Cells
한국생물공학회 학술대회
2022 .04
-
In Vivo Safety and Regeneration of Long-Term Transported Amniotic Fluid Stem Cells for Renal Regeneration
조직공학과 재생의학
2019 .01
-
Effect of Substrate Patterns on Cardiomyogenic Differentiation of Human Mesenchymal Stem cells
대한기계학회 춘추학술대회
2015 .05
-
Development of Novel Suspending Cell Culture Substrates for ex vivo Cell-gene Therapy
한국생물공학회 학술대회
2018 .04
-
CD4+ cytotoxic T cells: an emerging effector arm of anti-tumor immunity
BMB Reports
2023 .03
-
Co-Culturing Cancer Cells and Normal Cells in a Biochip under Electrical Stimulation
BioChip Journal
2018 .01
-
In Vitro Osteogenic Differentiation of Human Mesenchymal Stem Cells from Jawbone Compared with Dental Tissue
조직공학과 재생의학
2017 .01
-
Autocrine stimulation of IL-10 is critical to the enrichment of IL-10-producing CD40hiCD5+ regulatory B cells in vitro and in vivo
BMB Reports
2015 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0