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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 249 - 256 (8page)
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초록· 키워드
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In this study, localized surface plasmon resonance (LSPR) effect was characterized for immuneassay to detect cortisol conjugated nanoparticles (NPs) using a cuvette-compatible Au NPs layered sensor chip. A neuro stress hormone, cortisol, as target molecule was conjugated to 2 nm, 5 nm, 30 nm, and 40 nm Au NPs or 14 nm zinc oxide (ZnO) NPs to enhance the LSPR detection signals as plasmonic coupling elements with respect to cortisol monoclonal antibody (c-Mab) of probe molecule, which was immobilized on the Au NPs layered sensor. The cortisol conjugated 2 nm and 5 nm Au NPs and 14 nm ZnO NPs, which were selectively immune- bound to the c-Mab coated Au NPs sensor, showed LSPR effects to induce absorbance increases under UV-Vis spectroscopy. Also, the cortisol conjugated 2 nm, 5 nm, 30 nm and 40 nm Au NPs could show disparate plasmonic coupling effects to shift maximum absorbance wavelength peaks. Even though the cortisol conjugated 14 nm ZnO NPs could show increased absorbance only, the conjugation of cortisol to both Au and ZnO NPs was proved to enhance direct LSPR detection of the cortisol, which could not be explicitly sensed by itself for direct LSPR signal.
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참고문헌 (19)
참고문헌 신청
Stevens, R.C. / 2008 / Detection of cortisol in saliva with a flow-filtered, portable surface plasmon resonance biosensor system / Anal. Chem. 80:6747~6751
Frasconi, M. / 2009 / Surface plasmon resonance immunosensor for cortisol and cortisone determination / Anal. Bioanal. Chem. 394:2151~2159
Mitchell, J.S. / 2009 / Rapid ultrasensitive measurement of salivary cortisol using nanolinker chemistry coupled with surface plasmon resonance detection / Analyst. 134:380~386
Yamaguchi, M. / 2013 / Immunosensor with fluid control mechanism for salivary cortisol analysis / Biosens. Bioelectron. 41:186~191
Tahara, Y. / 2014 / Development of indirect competitive immuno-assay method using SPR detection for rapid and highly sensitive measurement of salivary cortisol levels / Front. Bioeng. Biotecnol. 2:15
Frasconi, M. / 2009 / Surface plasmon resonance immunosensor for cortisol and cortisone determination / Anal. Bioanal. Chem. 394:2151~2159
Mitchell, J.S. / 2009 / Rapid ultrasensitive measurement of salivary cortisol using nanolinker chemistry coupled with surface plasmon resonance detection / Analyst. 134:380~386
Yamaguchi, M. / 2013 / Immunosensor with fluid control mechanism for salivary cortisol analysis / Biosens. Bioelectron. 41:186~191
Tahara, Y. / 2014 / Development of indirect competitive immuno-assay method using SPR detection for rapid and highly sensitive measurement of salivary cortisol levels / Front. Bioeng. Biotecnol. 2:15
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