지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 93 - 101 (9page)
이용수
초록· 키워드
오류제보하기
The oxygen tensions and matrix stiffness play important roles in regulating cell response to cytotoxic drugs. In recent years, single spatiotemporal oxygen or matrix stiffness gradient has been established by various technologies for cell studies. However, the synergistic effects of the two factors on tumor cells remained elusive. In this study, we created a highly integrated and easy-to-operate microfluidic device. It was capable of generating more continuous, linear, stable and diffusive hydrogel stiffness gradient over a well-defined oxygen gradient. Sodium hydroxide and pyrogallol were used to scavenge oxygen and generate oxygen gradient, skillfully avoiding the utilization of bulky pressurized gas cylinders and sophisticated flow control by conventional methods. Utilizing the newly developed microfluidic device, we successfully performed drug test with a hypoxia sensitive anti-cancer drug, triapazamine (TPZ), on A549 cells under perpendicular construction of oxygen and spatially hydrogel stiffness gradients. The cell experiment results demonstrated the matrix stiffness-dependent cell drug resistance and hypoxia-induced cytotoxicity of TPZ. In summary, the developed microfluidic device exhibits high potential in the study of matrix stiffness-dependent cell responses and oxygen-sensitive drug cytotoxicity. Furthermore, the design principle can be functionally extended to other dual factors system and will facilitate the study of cell response in complex physiological and pathological conditions.
목차
등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
Yang, W. / 2015 / A novel microfluidic platform for studying mammalian cell chemotaxis in different oxygen environments under zero-flow conditions / Biomicrofluidics 9:044121
McCord, A.M. / 2009 / Physiologic oxygen concentration enhances the stem-like properties of CD133+ human glioblastoma cells in vitro / Mol. Cancer Res. 7:489~497
Sant, S. / 2010 / Biomimetic gradient hydrogels for tissue engineering / Can. J. Chem. Eng. 88:899~911
Li, Y. / 2016 / A microfluidic chip of multiple-channel array with various oxygen tensions for drug screening / Microfluid. Nanofluid. 20:1~9
Chen, Y. / 2011 / Generation of oxygen gradients in microfluidic devices for cell culture using spatially confined chemical reactions / Lab Chip 11:3626~3633
McCord, A.M. / 2009 / Physiologic oxygen concentration enhances the stem-like properties of CD133+ human glioblastoma cells in vitro / Mol. Cancer Res. 7:489~497
Sant, S. / 2010 / Biomimetic gradient hydrogels for tissue engineering / Can. J. Chem. Eng. 88:899~911
Li, Y. / 2016 / A microfluidic chip of multiple-channel array with various oxygen tensions for drug screening / Microfluid. Nanofluid. 20:1~9
Chen, Y. / 2011 / Generation of oxygen gradients in microfluidic devices for cell culture using spatially confined chemical reactions / Lab Chip 11:3626~3633
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Microfluidic System for Rapid Antimicrobial Susceptibility Testing of Clinical Samples at Single Cell Level
한국생물공학회 학술대회
2023 .04
-
Oxygen Regulated Microfluidic System for Controlled Physiological Hypoxic Environment
한국생물공학회 학술대회
2018 .10
-
Hypoxia-on-a-chip, Adjustable Oxygen Concentration
한국생물공학회 학술대회
2022 .09
-
Hydrogel-integrated Microfluidic Systems for Advanced Stem Cell Engineering
BioChip Journal
2019 .01
-
Microfluidic Model to Investigate Liver and Kidney Interaction in Hypoxia-induced Condition
한국생물공학회 학술대회
2021 .04
-
Numerical study of oxygen transport characteristics in lead-bismuth eutectic for gas-phase oxygen control
Nuclear Engineering and Technology
2021 .07
-
Microfluidic platform for disease modeling and drug screening
대한기계학회 춘추학술대회
2021 .08
-
복합자극에 따른 세포 반응 연구를 위한 미세유체 칩 개발
한국정밀공학회 학술발표대회 논문집
2016 .05
-
Microfluidic system for easy monitoring of drug delivery and cytotoxicity
한국고분자학회 학술대회 연구논문 초록집
2019 .04
-
Effects of Stiffness of Collagen-coated and Hydrogel Substrates on Cell-cell Interaction
한국고분자학회 학술대회 연구논문 초록집
2023 .04
-
Development of Oxygen-Carrying Nanoparticle for Bone regeneration
한국생물공학회 학술대회
2021 .10
-
Linear Chemical Gradient Generation in Microfluidic Channel by Flow Rate Control
한국정밀공학회 학술발표대회 논문집
2022 .05
-
Oxygen and Anticancer Drug Co-Delivery to Tumor Microenvironment via Targeted Oxygen Nanosomes
한국생물공학회 학술대회
2024 .09
-
Effect of Gas Diffusion Layer on La0.8Sr0.2CoO₃ Bifunctional Electrode for Oxygen Reduction and Evolution Reactions in an Alkaline Solution
한국수소및신에너지학회논문집
2016 .12
-
Trophoblast Invasion to Maternal Artery in Oxygen Regulated Microfluidic Chip
한국생물공학회 학술대회
2019 .10
-
High-throughput injection molded microfluidic device for single-cell analysis of spatiotemporal dynamics
대한기계학회 춘추학술대회
2021 .04
-
Formation and Validation of Two Orthogonal Concentration Gradients in Microfluidic-based Antibiotic Testing Platform
대한기계학회 춘추학술대회
2018 .04
-
Simple Paper-based Liver Cell Model for Drug Screening
BioChip Journal
2020 .01
-
Microfluidic stem cell engineering platform for cancer behavior analysis
한국생물공학회 학술대회
2020 .10
-
하이드로겔과 미세유체채널을 이용한 단속적 약물 공급소자
한국생산제조학회지
2019 .06
이 논문의 저자 정보
최근 본 자료
댓글(0)
0