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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 81 - 87 (7page)
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This paper describes continuous sorting and washing of cancer cells from blood samples by three-dimensional rotating flows and hydrophoretic cell focusing in a microchannel with slant trenches. Sample preparation for analysis of cancer cells such as sorting and washing is an important pre-requisite for their downstream analysis (i.e. enumeration or molecular characterization of cancer cells). Current cell separators, however, provide the capability of either sorting or washing. Washing process is routinely performed off-chip, preventing the automation of cellbased assays and point-of-care diagnostics. We present here a microfluidic method for on-chip cell sorting and washing. Slant trenches on a channel floor generate rotational flow streams and enable manipulation of the streams to passively cross cancer cells which remain focused in their equilibrium position by hydrophoresis. Using this device, we sorted cancer cells from contaminant particles and simultaneously washed them with a clean buffer, achieving high blood cell rejection efficiency of 99.3% and washing efficiency of 99.0%. The device enables simultaneous sorting and washing of cancer cells which can facilitate high-performance, automated cancer cell analysis.
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참고문헌 (26)
참고문헌 신청
Hyun, K.-A. / 2014 / Advances and critical concerns with the microfluidic enrichments of circulating tumor cells / Lab Chip 14:45~56
Nagrath, S. / 2007 / Isolation of rare circulating tumour cells in cancer patients by microchip technology / Nature 450:1235~1239
Yu, M. / 2014 / Ex vivo culture of circulating breast tumor cells for individualized testing of drug susceptibility / Science 345:216~220
Magbanua, M.J.M. / 2013 / Isolation of circulating tumor cells by immunomagnetic enrichment and fluorescence-activated cell sorting (IE/FACS) for molecular profiling / Methods 64:114~118
Jo, S.-M. / 2014 / Simple and efficient capture of EGFR-expressing tumor cells using magnetic nanoparticles / Sens. Actuator B: Chem 201:114~152
Nagrath, S. / 2007 / Isolation of rare circulating tumour cells in cancer patients by microchip technology / Nature 450:1235~1239
Yu, M. / 2014 / Ex vivo culture of circulating breast tumor cells for individualized testing of drug susceptibility / Science 345:216~220
Magbanua, M.J.M. / 2013 / Isolation of circulating tumor cells by immunomagnetic enrichment and fluorescence-activated cell sorting (IE/FACS) for molecular profiling / Methods 64:114~118
Jo, S.-M. / 2014 / Simple and efficient capture of EGFR-expressing tumor cells using magnetic nanoparticles / Sens. Actuator B: Chem 201:114~152
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