New Bone Formation in Tuberculous-Infected Vertebral Body Defect after Administration of Bone Marrow Stromal Cells in Rabbit Model

Ahmad Jabir Rahyussalim; Tri Kurniawati; Nurjati Chairani Siregar; Agus Syahrurachman; Ismail Hadisubroto Dilogo; Diah Iskandriati; Arni Diana Fitri

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자료유형: 학술저널

저자정보: Ahmad Jabir Rahyussalim (University of Indonesia) Tri Kurniawati (University of Indonesia) Nurjati Chairani Siregar (University of Indonesia) Agus Syahrurachman (University of Indonesia) Ismail Hadisubroto Dilogo (University of Indonesia) Diah Iskandriati (Bogor Agricultural Institute) Arni Diana Fitri (Bogor Agricultural Institute)

저널정보: 대한척추외과학회 Asian Spine Journal Asian Spine Journal Vol.10 No.1

발행연도: 2016.1

수록면: 1 - 5 (5page)

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Study Design: Preliminary experimental study using a rabbit spondylitis model. Purpose: To observe the ossification in a micro-environment containing live Mycobacterium tuberculosis transplanted with bone marrow stromal cells (BMSCs) in rabbits. Overview of Literature: BMSCs differentiate to osteoblasts and then osteocytes during ossification. Mycobacterium tuberculosis does not affect BMSC growth in vitro . Methods: Six rabbits were divided into two groups of three rabbits. One group was positive for spondylitis tuberculosis by culture, polymerase chain reaction (PCR), and histopathologically. The other group was positive by PCR and histopathologically. Both groups were treated using BMSC transplantation and anti-tuberculosis drugs. After 6 weeks, ossification was evaluated by enumerating the number of osteoblasts, osteocytes, and lesion level of calcium. Results: Mean number of osteoblasts was 207.00±31.00 in the first group and 220.33±73.46 in the second group. Mean number of intra-lesions osteocytes was in the first and second group was 18.33±30.04 and 31.00±26.87, respectively. Mean calcium level in the first group and second group was 2.94%±0.89% and 2.51%±0.13%, respectively. Total ossification score in the first and second group was 31.00 and 25.67, respectively. Conclusions: Mycobacterium tuberculosis provides support for new bone formation by stimulating intra-lesion calcium metabolism. The microscopic environment containing live Mycobacterium tuberculosis enhances ossification.

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