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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 508 - 525 (18page)
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초록· 키워드
오류제보하기
Astrocyte is the most abundant cell type in the central nervous system and its importance has been increasingly recognized in the brain pathophysiology. To study in vivo function of astrocyte, astrocyte-specific gene-targeting is regarded as a powerful approach. Especially, hGFAP-CreERT2, which expresses tamoxifen-inducible Cre recombinase under the human GFAP promoter, has been developed and characterized from several research groups. However, one of these mouse lines, [Tg(GFAP-Cre/ERT2)13Kdmc] from Ken McCarthy group has not been quantitatively analyzed, despite its frequent use. Here, we performed comprehensive characterization of this mouse line with quantitative analysis. By crossing this mouse line with Ai14 (RCL-tdTomato), a very sensitive Cre reporter mouse line, we visualized the Cre-expressing cells in various brain regions. For quantitative analysis, we immunostained S100β as an astrocytic marker and NeuN, tyrosine hydroxylase or calbindin as a neuronal marker in different brain regions. We calculated ‘astrocyte specificity’ as the proportion of co-labelled S100β and tdTomato positive cells in the total number of tdTomato positive cells and the ‘astrocyte coverage’ as the proportion of co-labelled S100β and tdTomato positive cells in the total number of S100β positive cells. Interestingly, we found varying degree of astrocyte specificity and coverage in each brain region. In cortex, hypothalamus, substantia nigra pars compacta and cerebellar Purkinje layer, we observed high astrocyte specificity (over 89%) and relatively high astrocyte coverage (over 70%). In striatum, hippocampal CA1 layer, dentate gyrus and cerebellar granule layer, we observed high astrocyte specificity (over 80%), but relative low astrocyte coverage (50-60%). However, thalamus and amygdala showed low astrocyte specificity (about 65%) and significant neuron specificity (over 30%). This hGFAP-CreERT2 mouse line can be useful for genetic modulations of target gene either in gain-of-function or loss-of-function studies in the brain regions with high astrocyte specificity and coverage. However, the use of this mouse line should be restricted to gain-of-function studies in the brain regions with high astrocyte specificity but low coverage. In conclusion, hGFAP-CreERT2 mouse line could be a powerful tool for gene-targeting of astrocytes in cortex, striatum, hippocampus, hypothalamus, substantia nigra pars compacta and cerebellum, but not in thalamus and amygdala.
목차
등록된 정보가 없습니다.
참고문헌 (38)
참고문헌 신청
Kaneko N / 2017 / Mechanisms of neuronal migration in the adult brain / J Neurochem 141:835~847
Goubard V / 2011 / Contribution of astrocytic glutamate and GABA uptake to corticostriatal information processing / J Physiol 589:2301~2319
Porter JT / 1997 / Astrocytic neurotransmitter receptors in situ and in vivo / Prog Neurobiol 51:439~455
Beltrán-Castillo S / 2017 / D-serine released by astrocytes in brainstem regulates breathing response to CO2levels / Nat Commun 8:838
Guthrie PB / 1999 / ATP released from astrocytes mediates glial calcium waves / J Neurosci 19:520~528
Goubard V / 2011 / Contribution of astrocytic glutamate and GABA uptake to corticostriatal information processing / J Physiol 589:2301~2319
Porter JT / 1997 / Astrocytic neurotransmitter receptors in situ and in vivo / Prog Neurobiol 51:439~455
Beltrán-Castillo S / 2017 / D-serine released by astrocytes in brainstem regulates breathing response to CO2levels / Nat Commun 8:838
Guthrie PB / 1999 / ATP released from astrocytes mediates glial calcium waves / J Neurosci 19:520~528
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