지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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Although Semisulcospira libertina is generally regarded as a supplement for the alleviation of alcohol hangover, little is known about its effects on cell metabolism. Therefore, this study was conducted to analyze the constituents of the extracts prepared using different extraction methods and to compare their biochemical properties. The amino acid contents were found to be much higher in acidic and enzymatic hydrolysates than hot water extracts from S. libertina. DPPH radical scavenging activities in acidic and enzymatic hydrolysates were higher than those of hot water extracts. Three types of S. libertina hydrolysate was added to HepG2 cells damaged by acetaminophen (AAP), after which the survival rate of HepG2 cell were measured. In addition, lactate dehydrogenase (LDH) activities in the culture media were evaluated. The survival rates of HepG2 cells were 77.0±4.3% and 81.5±1.3% at 3 h and 5h enzymatic hydrolysates, respectively. These cell survival rates were higher compared to those of the negative control group (67.8±4.3%) treated only with acetaminophen. Cellular toxicities induced by treatment with AAP were also significantly alleviated in response to treatment with the extracts of S. libertina. In addition, the activities of 2 key enzymes that metabolize ethanol, alcohol dehydrogenase and aldehyde dehydrogenase, were upregulated by 4.7- and 2.7-fold respectively in response to treatment with a 3 h enzymatic hydrolysate of S. libertina. Taken together, these results provide biochemical evidence of the method by which S. libertina exerts its biological functions, including the alleviation of alcohol hangover and the protection of liver cells against toxic insults.
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참고문헌 (29)
참고문헌 신청
Blois MS / 1958 / Antioxidant determinations by the use of a stable free radical / Nature 181:1199~1200
Cha HO / 1999 / Effect of Semisulcospira libertina, Corbicula sp. and Cipangopaludina chinensis malleata extracts on liver function and other biological activities / 45th National Sci. Exhibition of Ministry of Science and Technology
차재영 / 2009 / 알코올 대사 효소 alcohol dehydrogenase (ADH) 및 acetaldehyde dehydrogenase (ALDH) 활성에 미치는 아미노산의 영향 / 생명과학회지 19(9):1321~1327
Grunnet N / 1986 / The effect of ethanol on the betaoxidation of fatty acids / Alcohol. Clin. Exp. Res 10:64S~68S
Di Luzio N / 1967 / Role of lipid peroxidation in the pathogenesis of the ethanol-induced fatty liver / Fed. Proc 26:1436~1442
Cha HO / 1999 / Effect of Semisulcospira libertina, Corbicula sp. and Cipangopaludina chinensis malleata extracts on liver function and other biological activities / 45th National Sci. Exhibition of Ministry of Science and Technology
차재영 / 2009 / 알코올 대사 효소 alcohol dehydrogenase (ADH) 및 acetaldehyde dehydrogenase (ALDH) 활성에 미치는 아미노산의 영향 / 생명과학회지 19(9):1321~1327
Grunnet N / 1986 / The effect of ethanol on the betaoxidation of fatty acids / Alcohol. Clin. Exp. Res 10:64S~68S
Di Luzio N / 1967 / Role of lipid peroxidation in the pathogenesis of the ethanol-induced fatty liver / Fed. Proc 26:1436~1442
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