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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 985 - 998 (14page)
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초록· 키워드
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Normal and the spontaneous spirally rolled leaves of Cymbidium goeringii var. longibracteatum were used for RNA sequencing analyses using the Illumina paired-end sequencing technique to figure out the differently- expressed genes in two samples. About 5.65 and 4.82 Gb sequencing data of raw reads were obtained from 2 cDNA libraries of normal and the spirally rolled leaves respectively. After data filtering, quality checks and de novo assembly, a total of 48,935 unigenes with an average sequence length of 820 nt were generated. In addition, the transcriptome change in normal and the spirally rolled leaves was investigated. With non-redundant annotation, 219 differentially expressed genes (DEGs) are identified, with 147 up-regulated genes and 72 down-regulated genes. Out of these DEGs, 21 DEGs (9.59 %) were involved in cell wall modeling enzymes, such as expansin, xyloglucan endo-transglycosylase, pectate lyase, cell wall-associated hydrolase. Besides, other DEGs were predominantly classified as genes involved in transcription factor and signal sense and transduction signaling. This study presents the first comprehensive characterization of the leave transcriptomes of Cymbidium goeringii var. longibracteatum. This study not only gave us valuable sequence resources of this species, but also provided theoretical foundation for cultivar breeding of leaf mutation in C. goeringii var. longibracteatum.
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참고문헌 (42)
참고문헌 신청
Benjamini Y / 2001 / The control of the false discovery rate in multiple testing under dependency / Ann Stat 29:1165~1188
Berna´ G / 1999 / A mutational analysis of leaf morphogenesis in Arabidopsis thaliana / Genetics 152:729~742
Chen H / 2013 / Sequencing and de novo assembly of the Asian clam (Corbicula fluminea) transcriptome using the Illumina GAIIx method / PLoS ONE 8:e79516
Chen Y / 2014 / Transcriptome analysis of differentially expressed genes relevant to variegation in peach flowers / PLoS One 9:e90842
Conesa A / 2005 / Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research / Bioinformatics 21:3674~3676
Berna´ G / 1999 / A mutational analysis of leaf morphogenesis in Arabidopsis thaliana / Genetics 152:729~742
Chen H / 2013 / Sequencing and de novo assembly of the Asian clam (Corbicula fluminea) transcriptome using the Illumina GAIIx method / PLoS ONE 8:e79516
Chen Y / 2014 / Transcriptome analysis of differentially expressed genes relevant to variegation in peach flowers / PLoS One 9:e90842
Conesa A / 2005 / Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research / Bioinformatics 21:3674~3676
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